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Western blot analysis of HDAC2 in HeLa cell lysate with HDAC2 antibody at (A) 0.5 and (B) 1 μg/ml.
Immunohistochemistry of HDAC2 in rat testis tissue with HDAC2 antibody at 5 μg/mL.
Immunofluorescence of HDAC2 in rat testis tissue with HDAC2 antibody at 20 μg/mL.
Western blot analysis of HDAC2 in HeLa cell lysate with HDAC2 antibody at (A) 0.5 and (B) 1 μg/ml.
Trial Size

HDAC2 Antibody

CATALOG NUMBER: 7899

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Homology:
Predicted species reactivity based on immunogen sequence: Chicken: (94%)
Immunogen:
HDAC2 antibody was raised against an 18 amino acid peptide near the carboxy terminus of human HDAC2.
The immunogen is located within the last 50 amino acids of HDAC2.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
HDAC2 antibody can be used for detection of HDAC2 by Western blot at 0.5 - 1 μg/ml. Antibody can also be used for Immunohistochemistry at 5 μg/mL. For Immunoflorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested.
Specificity:
HDAC2 antibody is human, mouse and rat reactive. At least two isoforms of HDAC2 are known to exist; this antibody will detect both isoforms. HDAC2 antibody is predicted to not cross-react with other members of the HDAC family.
Positive Control 1:
Cat. No. 1201 - HeLa Cell Lysate
Predicted Molecular Weight:
Predicted: 54 kDa
Observed: 52 kDa
Purification:
HDAC2 antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
HDAC2 antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
HDAC2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Ncbi Official Symbol:
HDAC2
Additional Names:
HDAC2 Antibody: HD2, RPD3, YAF1, Histone deacetylase 2, HD2
Protein Accession Number:
NP_001518
Protein Gi Number:
293336691
Ncbi Gene Id Number:
3066
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The histone deacetylase (HDAC) family contains multiple members which are divided into four classes. Class I of the HDAC family comprises four members, HDAC1, 2, 3, and 8, each of which contains a deacetylase domain and exhibits a different, individual substrate specificity and function in vivo (1). HDAC2 was first identified as as a mammalian homolog to the yeast transcriptional regulator RPD3 (2). HDAC2 forms transcriptional repressor complexes by associating with many different proteins, including YY1, a mammalian zinc-finger transcription factor (2,3). Thus, it plays an important role in transcriptional regulation, cell cycle progression and developmental events (4).
Background Reference 1:
Taunton J, Hassig CA, and Schreiber SL. A mammalian histone deacetylase related to the yeast transcriptional regulator Rpd3p. Science 1996; 272:408-11.
Background Reference 2:
Yang WM, Inouye C, Zeng Y, et al. Transcriptional repression by YY1 is mediated by interaction with a mammalian homolog of the yeast global regulator RPD3. Proc. Natl. Acad. Sci. USA 1996; 93:12845-50.
Background Reference 3:
Cress WD and Seto E. Histone deacetylases, transcriptional control, and cancer. J. Cell Phys. 2000; 184:1-16.
Background Reference 4:
Kramer OH. HDAC2: a critical factor in health and disease. Trends Pharmacol. Sci. 2009; 30:647-55.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

7899

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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