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Western blot analysis of a His-tag-containing recombinant protein with His-tag antibody at (A) 0.25 and (B) 0.5 μg/ml.
Western blot analysis of 20ng of His-tagged recombinant GGP1 protein using 1 μg of His antibody to immunoprecipitate and 1 μg/ml anti-GGP1 antibody to detect.
Western blot analysis of a His-tag-containing recombinant protein with His-tag antibody at (A) 0.25 and (B) 0.5 μg/ml.

His-tag Antibody

CATALOG NUMBER: 7877

Clonality:
Polyclonal
Tested Applications:
ELISA, IP, WB
Host Species:
Rabbit
Species Reactivity:
Tag
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Tag
Immunogen:
His-tag antibody was raised against a synthetic peptide containing HHHHHH.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IP, WB
Application Note:
His-tag antibody can be used for detection of His-tag epitope-containing recombinant proteins by Western blot at 0.5 - 1 μ/ml.
Specificity:
His-tag antibody will detect amino- or carboxy-terminal His-tagged recombinant proteins.
Predicted Molecular Weight:
N/A
Purification:
His-tag antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
His-tag antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
His-tag antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Ncbi Official Symbol:
N/A
Additional Names:
His-tag, His, 6xHis
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Epitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. His-tag is one of the more popular epitope tags for expressed recombinant proteins.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

7877

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.

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Bulk Pricing Available

Custom Formulations: azide free, endotoxin testing & endotoxin removal.

Pair your antibody with a Lysate, Peptide, Control, & /OR Secondary Antibody.

Contact our Customer Care Team for more information

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