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Western blot analysis of TRIP12 in rat colon tissue lysate with TRIP12 antibody at 1 μg/ml.
Immunohistochemistry of TRIP12 in rat colon tissue with TRIP12 antibody at 5 μg/ml.
Immunofluorescence of TRIP12 in rat colon tissue with TRIP12 antibody at 20 μg/ml.
Immunocytochemistry of TRIP12 in Hela cells with TRIP12 Antibody at 2 μg/ml.
Western blot analysis of TRIP12 in rat colon tissue lysate with TRIP12 antibody at 1 μg/ml.
Trial Size

TRIP12 Antibody

CATALOG NUMBER: 7953

Clonality:
Polyclonal
Tested Applications:
ELISA, ICC, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (100%)
Immunogen:
TRIP12 antibody was raised against a 18 amino acid peptide near the carboxy terminus of human TRIP12.
The immunogen is located within amino acids 1930 - 1980 of TRIP12.
Conjugate:
Unconjugated
Tested Applications:
ELISA, ICC, IF, IHC-P, WB
Application Note:
TRIP12 antibody can be used for detection of TRIP12 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL. For immunocytochemistry start at 2 μg/mL.For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in rat samples; Immunohistochemistry in rat samples; Immunocytochemistry in human samples; and Immunofluorescence in rat samples. All other applications and species not yet tested.
Specificity:
TRIP12 antibody is human, mouse and rat reactive. At least four isoforms are known to exist.
Positive Control 1:
Cat. No. 1472 - Rat Colon Tissue Lysate
Predicted Molecular Weight:
Predicted: 219 kDa
Observed: 220 kDa
Purification:
TRIP12 antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
TRIP12 antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
TRIP12 antibody can be can be stored at 4°C, stable for up to one year. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
TRIP12
Additional Names:
TRIP12 Antibody: ULF, TRIP-12, KIAA0045, ULF, E3 ubiquitin-protein ligase TRIP12, E3 ubiquitin-protein ligase for Arf
Protein Accession Number:
NP_004229
Protein Gi Number:
10863903
Ncbi Gene Id Number:
9320
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Thyroid hormone receptors (TRs) are transcription factors that regulate the expression of specific genes in a hormone-dependent manner (1). TRIP12 (thyroid hormone receptor interactor 12) is an ATP-dependent E3 ubiquitin ligase involved in the human ubiquitin fusion degradation (UFD) pathway and also modulates the NEDD8 pathway (2,3). TRIP12 contains one WWE domain and a single HECT (E6AP-type E3 ubiquitin-protein ligase) domain suggested to contain a noncovalent ubiquitin-binding site (4). TRIP12 acts as a key regulator of DNA damage response and the ubiquitin ligase activity of TRIP12 is essential for mouse development (5).
Background Reference 2:
An CI, Ganio E, and Hagiwara N. Trip12, a HECT domain E3 ubiquitin ligase, targets Sox6 for proteasomal degradation and affects fiber type-specific gene expression in muscle cells. Skelet. Muscle 2013; 3:11.
Background Reference 3:
Poulsen EG, Steinhauer C, Lees M, et al. HUWE1 and TRIP12 collaborate in degradation of ubiquitin-fusion proteins and misframed ubiquitin. PLoS One. 2012; 7:e50548.
Background Reference 4:
Park Y, Yoon SK, and Yoon JB. The HECT domain of TRIP12 ubiquitinates substrates of the ubiquitin fusion degradation pathway. J. Biol. Chem. 2009; 284:1540-9.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

7953

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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