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Western blot analysis of LIN54 in HeLa cell lysate with LIN54 antibody at 1 μg/ml.
Immunocytochemistry of LIN54 in HeLa cells with LIN54 at 2.5 μg/mL.
Immunofluorescence of LIN54 in HeLa cells with LIN54 antibody at 20 μg/ml.
Immunocytochemistry of LIN54 in HeLa cells with LIN54 antibody at 10 μg/ml.
Immunofluorescence of LIN54 in HeLa cells with LIN54 antibody at 20 μg/ml.<br><br>Red: LIN54 Antibody (7933) <br> Blue: DAPI staining
Western blot analysis of LIN54 in HeLa cell lysate with LIN54 antibody at 1 μg/ml.
Trial Size

LIN54 Antibody

CATALOG NUMBER: 7933

Clonality:
Polyclonal
Tested Applications:
ELISA, ICC, IF, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
LIN54 antibody was raised against a 16 amino acid peptide near the carboxy terminus of human LIN54.
The immunogen is located within the last 50 amino acids of LIN54.
Conjugate:
Unconjugated
Tested Applications:
ELISA, ICC, IF, WB
Application Note:
LIN54 antibody can be used for detection of LIN54 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificity:
LIN54 antibody is human, mouse, and rat reactive. Multiple isoforms of LIN54 are known to exist.
Positive Control 1:
Cat. No. 1201 - HeLa Cell Lysate
Positive Control 2:
Cat. No. 17-001 - HeLa Cell Slide
Predicted Molecular Weight:
Predicted: 88 kDa
Observed: 82 kDa
Purification:
LIN54 antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
LIN54 antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
LIN54 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Ncbi Official Symbol:
LIN54
Additional Names:
LIN-54, JC8.6, CXCDC1, MIP120
Protein Accession Number:
NP_919258
Protein Gi Number:
169234719
Ncbi Gene Id Number:
132660
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
LIN54 is a component of the LIN, or DREAM, complex, an essential regulator of cell cycle genes. This complex functions as a transcriptional repressor by binding to promoters of E2F target genes that regulate the G1/S transition (1). LIN54 is required for cell cycle progression and can bind to the cdc2 promoter in a sequence-specific manner (2).
Background Reference 1:
Schmit F, Cremer S, and Gaubatz S. LIN54 is an essential core subunit of the DREAM/LINC complex that binds to the cdc2 promotoer in a sequence-specific manner. FEBS J. 2009; 276:5703-16.
Background Reference 2:
Matsuo T, Kuramoto H, Kumazaki T, et al. LIN54 harboring a mutation in CHC domain is localized to the cytoplasm and inhibits cell cycle progression. Cell Cycle 2012; 11:3227-36.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

7933

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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