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Western blot analysis of BATF3 in 293 cell lysate with BATF3 antibody at 0.5 μg/ml in (A) the absence and (B) the presence of blocking peptide.
Immunohistochemistry of BATF3 in human kidney tissue with BATF3 antibody at 5 μg/mL.
Immunofluorescence of BATF3 in human kidney tissue with BATF3 Antibody at 20 μg/mL.
Western blot analysis of BATF3 in 293 cell lysate with BATF3 antibody at 0.5 μg/ml in (A) the absence and (B) the presence of blocking peptide.
Trial Size

BATF3 Antibody

CATALOG NUMBER: 7939

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
BATF3 antibody was raised against a 16 amino acid peptide near the amino terminus of human BATF3.
The immunogen is located within the first 50 amino acids of BATF3.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
BATF3 body can be used for detection of BATF3 by Western blot at 0.5 μg/ml. Antibody can also be used for Immunohistochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificity:
BATF3 antibody is human, mouse and rat reactive. BATF3 antibody is predicted to have no cross-reactivity to other BATF family members.
Positive Control 1:
Cat. No. 1210 - HEK293 Cell Lysate
Predicted Molecular Weight:
Predicted: 14 kDa
Observed: 17 kDa
Purification:
BATF3 antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
BATF3 antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
BATF3 antibody can be stored at 4°C up to one year. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
BATF3
Additional Names:
Basic leucine zipper transcriptional factor ATF-like 3, SNFT, JDP1, JUNDM1
Protein Accession Number:
NP_061134
Protein Gi Number:
8924246
Ncbi Gene Id Number:
55509
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
BATF3, also known as SNFT, belongs to the basic leucine zipper transcriptional family and functions as a transcriptional repressor when heterodimerizing with Jun (1) and can repress the transcription of IL-2 and MMP-1 (1,2). BATF3 is required for the development of CD8alpha+ classical dendritic cells (DCs) and the related CD103+ DCs that cross-present antigens to CD8 T cells (3,4).
Background Reference 1:
Iacobelli M, Wachsman W, and McGuire KL. Repression of IL-2 promoter activity by the novel basic leucine zipper p21SNFT protein. J. Immunol. 2000; 154:860-8.
Background Reference 2:
Bower KE, Fritz JM, and McGuire KL. Transcriptional repression of MMP-1 by p21SNFT and reduced invasiveness of hepatocarcinoma cells. Oncogene 2004; 23:8805-14.
Background Reference 3:
Hildner K, Edelson BT, Purtha WE, et al. BATF3 deficiency reveals a critical role for CD8alpha+ dendritic cells in cytotoxic T cell immunity. Science 2008; 322:1097-1100.
Background Reference 4:
Edelson BT, Peripheral CD103+ dendritic cells form a unified subset developmentally related to CD8alpha+ conventional dendritic cells. J. Exp. Med. 2010; 207:823-36.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

7939

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.

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