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Western blot analysis of TXNIP in C2C12 cell lysate with TXNIP antibody at 0.5 μg/ml in (A) the absence and (B) the presence of blocking peptide.
Immunohistochemistry of TXNIP in human kidney tissue with TXNIP antibody at 2 μg/ml.
Immunofluorescence of TXNIP in human kidney tissue with TXNIP antibody at 5 μg/ml.<br><br>Red: TXNIP Antibody (7941) <br> Blue: DAPI staining
Western blot analysis of TXNIP in C2C12 cell lysate with TXNIP antibody at 0.5 μg/ml in (A) the absence and (B) the presence of blocking peptide.
Trial Size

TXNIP Antibody

CATALOG NUMBER: 7941

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Homology:
Predicted species reactivity based on immunogen sequence: Pig: (94%)
Immunogen:
TXNIP antibody was raised against a 16 amino acid peptide near the center of human TXNIP.
The immunogen is located within amino acids 110 - 160 of TXNIP.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
TXNIP body can be used for detection of TXNIP by Western blot at 0.5 - 1 μg/ml. Antibody can also be used for Immunohistochemistry at 2 μg/mL. For Immunoflorescence start at 5 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificity:
TXNIP antibody is human, mouse and rat reactive.
Positive Control 1:
Cat. No. 1285 - C2C12 Cell Lysate
Predicted Molecular Weight:
Predicted: 43 kDa
Observed: 39 kDa
Purification:
TXNIP antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
TXNIP antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
TXNIP antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Ncbi Official Symbol:
TXNIP
Additional Names:
Thioredoxin-interacting protein, BMP and activin membrane-bound inhibitor homolog, non-metastatic gene A protein, NMA
Protein Accession Number:
NP_006463
Protein Gi Number:
171184421
Ncbi Gene Id Number:
10628
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Thioredoxin-interacting protein (TXNIP) belongs to the arrestin family and plays a critical role in the antioxidant defense mechanisms of hematopoietic cells by activating the p53 pathway during oxidative stress (1,2). It functions as a transcriptional repressor and acts as an oxidative stress mediator by inhibiting thioredoxin activity (2). TXNIP expression is reduced in many types of tumors, and TXNIP overexpression inhibits tumor growth by blocking cell-cycle progression (3). It has recently reported that TXNIP deficiency correlates with a high incidence of hepatocellular carcinoma (HCC) (4). TXNIP and p53 interactions could potentially be a therapeutic target for oxidative stress-related diseases such as hematopoietic malignancies and metabolic diseases (5).
Background Reference 1:
Chen KS and DeLuca HF. Isolation and characterization of a novel cDNA from HL-60 cells treated with 1, 25-dihydroxyvitamin D-3. Biochim. Biophys. Acta. 1994; 1219:26-32.
Background Reference 2:
Jeong M, Piao ZH, Kim MS, et al. Thioredoxin-interacting protein regulates hematopoietic stem cell quiescence and mobilization under stress conditions. J. Immunol. 2009; 183:2495–505.
Background Reference 3:
Han SH, Jeon JH, Ju HR, et al. VDUP1 upregulated by TGF-beta1 and 1,25-dihydorxyvitamin D3 inhibits tumor cell growth by blocking cell-cycle progression. Oncogene 2003; 22:4035–46.
Background Reference 4:
Kwon HJ, Won YS, Yoon YD, et al. Vitamin D3 up-regulated protein 1 deficiency accelerates liver regeneration after partial hepatectomy in mice. J. Hepatol. 2011; 54:1168–76.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

7941

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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