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Western blot analysis of AKAP4 in human testis tissue lysate with AKAP4 antibody at (A) 1 and (B) 2 μg/ml.
Immunohistochemistry of AKAP4 in human testis tissue with AKAP4 antibody at 5 μg/ml.
Immunofluorescence of AKAP4 in human testis tissue with AKAP4 antibody at 20 μg/ml.
Western blot analysis of AKAP4 in human testis tissue lysate with AKAP4 antibody at (A) 1 and (B) 2 μg/ml.
Trial Size

AKAP4 Antibody

CATALOG NUMBER: 7961

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Rat: (94%), Mouse: (88%)
Immunogen:
AKAP4 antibody was raised against a 17 amino acid peptide near the amino terminus of human AKAP4.
The immunogen is located within the first 50 amino acids of AKAP4.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
AKAP4 antibody can be used for detection of AKAP4 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificity:
AKAP4 antibody is human specific. At least three isoforms are known to exist; this antibody will detect the two longest isoforms.
Positive Control 1:
Cat. No. 1313 - Human Testis Tissue Lysate
Positive Control 2:
Cat. No. 11-701 - Human Testis Tissue Slide
Predicted Molecular Weight:
Predicted: 94 kDa
Observed: 100 kDa
Purification:
AKAP4 antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
AKAP4 antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
AKAP4 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Ncbi Official Symbol:
AKAP4
Additional Names:
A-kinase anchor protein 4, AKAP-4, AKAP82, hAKAP82, CT99, FSC1, HI, PRKA4
Protein Accession Number:
NP_003877
Protein Gi Number:
21493037
Ncbi Gene Id Number:
8852
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins, which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell (1). AKAP4, also known as hAKAP82, is a major sperm fibrous sheath protein that localizes to the entire length of the flagellum of human sperm (2). It has been suggested that AKAP4 plays multiple roles in sperm motility and regulation of signal transduction pathways (2). AKAP4 has also been postulated to be a potential biomarker for breast cancer (3).
Background Reference 1:
Carnegie GK, Means CK, and Scott JD. A-kinase anchoring proteins: from protein complexes to physiology and disease. IUBMB 2009; 61:394-406.
Background Reference 2:
Turner RM, Johnson LR, Haig-Ladewig L, et al. An X-linked gene encodes a major human sperm fibrous sheath protein, hAKAP82. Genomic organization, protein kinase A-RII binding, and distribution of the precursor in the sperm tail. J. Biol. Chem. 1998; 273:32135-41.
Background Reference 3:
Saini S, Jagadish N, Gupta A, et al. A novel cancer testis antigen, A-kinase anchor protein 4 (AKAP4) is a potential biomarker for breast cancer. PLoS One 2013; 8:e57095.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

7961

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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