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Western blot analysis of RHOG in human kidney tissue lysate with RHOG antibody at 1 μg/ml.
Immunohistochemistry of RHOG in mouse kidney tissue with RHOG antibody at 5 μg/ml.
Western blot analysis of RHOG in human kidney tissue lysate with RHOG antibody at 1 μg/ml.
Trial Size

RHOG Antibody

CATALOG NUMBER: 7981

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (87%), Guinea pig: (87%), Chicken: (87%), Pig: (71%)
Immunogen:
RHOG antibody was raised against a 15 amino acid peptide near the amino terminus of human RHOG.
The immunogen is located within amino acids 40 - 90 of RHOG.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC-P, WB
Application Note:
RHOG antibody can be used for detection of RHOG by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL.
Antibody validated: Western Blot in human samples and Immunohistochemistry in mouse samples. All other applications and species not yet tested.
Specificity:
RHOG antibody is human, mouse and rat reactive.
Positive Control 1:
Cat. No. 1305 - Human Kidney Tissue Lysate
Predicted Molecular Weight:
Predicted: 21 kDa
Observed: 23 kDa
Purification:
RHOG antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
RHOG antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
RHOG antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Ncbi Official Symbol:
RHOG
Additional Names:
Ras homolog family member G, Rho-related GTP-binding protein, ARHG
Protein Accession Number:
NP_001656
Protein Gi Number:
46249393
Ncbi Gene Id Number:
391
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The ras homolog family member G (RHOG), also known as rho-related GTP-binding protein, is a member of the Rho family of small GTPases, which cycle between inactive GDP-bound and active GTP-bound states and function as molecular switches in signal transduction cascades (1). RHOG controls a pathway that requires the microtubule network and activates Rac1 and Cdc42Hs independently of their growth factor signaling pathway (2). RHOG is also involved in the transcriptional regulation of interferon-gamma and nuclear factor of activated T cells (NFAT) and the regulation of the actin skeleton in lymphocytes (3).
Background Reference 1:
Vincent S, Janteur P, and Fort P. Growth-regulated of rhoG, a new member of the ras homolog family. Mol. Cell Biol. 1992; 12:3138-48.
Background Reference 2:
Gauthier-Rouviere C, Vignal E, Meriane M, et al. RhoG GTPase controls a pathway that independently activates Rac1 and Cdc42Hs. Mol. Biol. Cell 1998; 9:1379-94.
Background Reference 3:
Vigorito E, Billadeu DD, Savoy D, et al. RhoG regulates gene expression and the actin skeleton in lymphocytes. Oncogene 2003; 22:330-42.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

7981

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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