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Western blot analysis of P2RX7 in 3T3 cell lysate with P2RX7 antibody at (A) 1 and (B) 2 μg/ml.
Immunocytochemistry of P2RX7 in 3T3 cells with P2RX7 antibody at 5 μg/mL.
Immunofluorescence of P2RX7 in 3T3 cells with P2RX7 antibody at 20 μg/mL.
Western blot analysis of P2RX7 in 3T3 cell lysate with P2RX7 antibody at (A) 1 and (B) 2 μg/ml.
Trial Size

P2RX7 Antibody

CATALOG NUMBER: 8015

Clonality:
Polyclonal
Tested Applications:
ELISA, ICC, IF, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Homology:
Predicted species reactivity based on immunogen sequence: Rat: (81%)
Immunogen:
P2RX7 antibody was raised against a 17 amino acid peptide near the carboxy terminus of human P2RX7.
The immunogen is located within the last 50 amino acids of P2RX7.
Conjugate:
Unconjugated
Tested Applications:
ELISA, ICC, IF, WB
Application Note:
P2RX7 antibody can be used for detection of P2RX7 by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunocytochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunocytochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Specificity:
P2RX7 antibody is human, mouse, and rat reactive. Multiple isoforms of P2RX7 are known to exist.
Positive Control 1:
Cat. No. 1212 - 3T3 Cell Lysate
Positive Control 2:
Cat. No. 17-201 - 3T3/BALB Cell Slide
Predicted Molecular Weight:
Predicted: 34, 47, 65 kDa
Observed: 54 kDa
Purification:
P2RX7 antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
P2RX7 antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
P2RX7 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Ncbi Official Symbol:
P2RX7
Additional Names:
P2X purinoceptor 7, P2X7, purinergic receptor P2X ligand-gated ion channel 7
Protein Accession Number:
NP_002553
Protein Gi Number:
300068987
Ncbi Gene Id Number:
5027
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The purinergic receptor P2X ligand-gated ion channel 7 (P2RX7) belongs to the family of purinoceptors for ATP (1). This receptor functions as a ligand-gated ion channel and is responsible for ATP-dependent lysis of macrophages through the formation of membrane pores permeable to large molecules (1,2). Activation of this nuclear receptor by ATP in the cytoplasm may be a mechanism by which cellular activity can be coupled to changes in gene expression (2). Recent studies have suggested that P2RX7 may play a key role in immune-mediated diseases such as rheumatoid arthritis (3) as well as neuropsychiatric disorders (4).
Background Reference 1:
Surprenant A, Rassendren F, Kawashima E, et al. The cytosolic P2Z receptor for extracellular ATP identified as a P2X receptor (P2X7). Science 1996; 272:735-8.
Background Reference 2:
North RA. Molecular physiology of P2X receptors. Physiol. Rev. 2002; 82:1013-67.
Background Reference 3:
Labasi JM, Petrushova N, Donovan C, et al. Absence of the P2X7 receptor alters leukocyte function and attenuates an inflammatory response. J. Immunol. 168:6436-45.
Background Reference 4:
Basso AM, Bratcher NA, Harris RR, et al. Behavioral profile of P2X7 receptor knockout mice in animal models of depression and anxiety; relevance for neuropsychiatric disorders. Behav. Brain Res. 2009; 198:83-90.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

8015

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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