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Western blot analysis of NOSTRIN in SW480 cell lysate with NOSTRIN antibody at (A) 1 and (B) 2 μg/ml.
Immunohistochemistry of NOSTRIN in mouse kidney tissue with NOSTRIN antibody at 5 μg/ml.
Immunofluorescence of NOSTRIN in mouse kidney tissue with NOSTRIN antibody at 20 μg/ml.
Western blot analysis of NOSTRIN in SW480 cell lysate with NOSTRIN antibody at (A) 1 and (B) 2 μg/ml.
Trial Size

NOSTRIN Antibody

CATALOG NUMBER: 8039

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (94%)
Immunogen:
NOSTRIN antibody was raised against a 17 amino acid peptide near the carboxy terminus of human NOSTRIN.
The immunogen is located within amino acids 430 - 480 of NOSTRIN.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
NOSTRIN antibody can be used for detection of NOSTRIN by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Specificity:
NOSTRIN antibody is human, mouse and rat reactive. At least four isoforms are known to exist; this antibody will detect all four isoforms.
Positive Control 1:
Cat. No. 1221 - SW480 Cell Lysate
Predicted Molecular Weight:
Predicted: 47, 53, 56, 62 kDa
Observed: 68 kDa
Purification:
NOSTRIN antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
NOSTRIN antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
NOSTRIN antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Ncbi Official Symbol:
NOSTRIN
Additional Names:
Nitric oxide synthase traffic inducer, eNOS-trafficking inducer, Nitric oxide synthase trafficker, BM247 homolog, DaIP2
Protein Accession Number:
NP_443178
Protein Gi Number:
284172479
Ncbi Gene Id Number:
115677
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
NOSTRIN. also known as endothelial nitric oxide synthase traffic inducer, is a member of the Pombe Cdc15 homology (PCH) family of proteins which may function as the modulator of the subcellular localisation and activity of endothelial nitric oxide synthase (eNOS) (1,2). It decreases NOS3 activity by inducing its translocation away from the plasma membrane (3). NOSTRIN typically exists as a trimer and is expressed in the vascular endothelial cells of highly vascularized tissues such as placenta, lung, kidney and heart. NOSTRIN is necessary for proper vascular development in zebrafish and postnatal retinal angiogenesis in mice (4,5).
Background Reference 1:
Zimmermann K, Opitz N, Dedio J, et al. NOSTRIN: a protein modulating nitric oxide release and subcellular distribution of endothelial nitric oxide synthase. Proc. Natl. Acad. Sci. USA 2002; 99:17167-72.
Background Reference 2:
Icking A, Matt S, Opitz N, et al. NOSTRIN functions as a homotrimeric adaptor protein facilitating internalization of eNOS. J. Cell Sci. 2005; 118:5059-69.
Background Reference 3:
Schilling K, Opitz N, Wiesenthal A, et al. Translocation of endothelial nitric-oxide synthase involves a ternary complex with caveolin-1 and NOSTRIN. Mol. Biol. Cell 2006; 17:3870-80.
Background Reference 4:
Icking A, Schilling K, Wiesenthal A, et al. FCH/Cdc15 domain determines distinct subcellular localization of NOSTRIN. FEBS Lett. 2006; 580:223-8.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

8039

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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