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Western blot analysis of DTX4 in HeLa cell lysate with DTX4 antibody at 1 μg/ml.
Immunohistochemistry of DTX4 in human spleen tissue with DTX4 antibody at 5 μg/ml.
Immunofluorescence of DTX4 in human spleen tissue with DTX4 antibody at 20 μg/ml.
Western blot analysis of DTX4 in HeLa cell lysate with DTX4 antibody at 1 μg/ml.
Trial Size

DTX4 Antibody

CATALOG NUMBER: 8057

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
DTX4 antibody was raised against a 19 amino acid peptide near the center of human DTX4.
The immunogen is located within amino acids 230 - 280 of DTX4.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
DTX4 antibody can be used for detection of DTX4 by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificity:
DTX4 antibody is human and mouse reactive. At least two isoforms of DTX4 are known to exist; this antibody will detect both isoforms. DTX4 antibody is predicted to not cross-react with other DTX protein family members.
Positive Control 1:
Cat. No. 1201 - HeLa Cell Lysate
Positive Control 2:
Cat. No. 10-901 - Human Spleen Tissue Slide
Predicted Molecular Weight:
Predicted: 68 kDa
Observed: 68 kDa
Purification:
DTX4 antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
DTX4 antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
DTX4 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Ncbi Official Symbol:
DTX4
Additional Names:
Deltex 4 E3 ubiquitin ligase, RNF155
Protein Accession Number:
NP_055992
Protein Gi Number:
148237498
Ncbi Gene Id Number:
23220
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The Deltex 4 E3 ubiquitin ligase (DTX4) is a member of a protein family that is involved in Notch signaling and neurogenesis (1). Recent studies have shown that DTX4 is also associated with the innate immune response (2). Following the activation of type I interferon signaling, the Nod-like receptor protein 4 (NLRP4) recruits DTX4 for Lys48-linked polyubiquitination and degradation of the kinase TBK1 (NAK), thereby maintaining immune homeostasis during antiviral innate immunity (2).
Background Reference 1:
Kishi N, Tang Z, Maeda Y, et al. Murine homologs of deltex define a novel gene family involved in vertebrate Notch signaling and neurogenesis. Int. J. Dev. Neurosci. 2001; 19:21-35.
Background Reference 2:
Cui J, Li Y, Zhu L, et al. NLRP4 negatively regulated type I interferon signaling by targeting the kinase TBK1 for degradation via the ubiquitin ligase DTX4. Nat. Immunol. 2012; 13:387-95.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

8057

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

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