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Western blot analysis of MYH3 in fetal human skeletal muscle lysate with MYH3 antibody at 1 μg/ml.
Immunohistochemistry of MYH3 in mouse skeletal muscle tissue with MYH3 antibody at 5 μg/ml.
Western blot analysis of MYH3 in fetal human skeletal muscle lysate with MYH3 antibody at 1 μg/ml.
Trial Size

MYH3 Antibody

CATALOG NUMBER: 8087

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
MYH3 antibody was raised against a 19 amino acid peptide near the amino terminus of human MYH3.
The immunogen is located within amino acids 30 - 80 of MYH3.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC-P, WB
Application Note:
MYH3 antibody can be used for detection of MYH3 by Western blot at 1 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL.
Antibody validated: Western Blot in human samples and Immunohistochemistry in mouse samples. All other applications and species not yet tested.
Specificity:
MYH3 antibody is human, mouse and rat reactive. MYH3 antibody is predicted to not cross-react with other members of the myosin heavy chain family.
Positive Control 1:
Cat. No. XBL-10413 - Fetal Human Skeletal Muscle Lysate
Predicted Molecular Weight:
Predicted: 240 kDa
Observed: 245 kDa
Purification:
MYH3 antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
MYH3 antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
MYH3 antibody can be stored at 4°C, stable for up to one year. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
MYH3
Additional Names:
Myosin heavy chain 3, Myosin-3, Myosin heavy chain skeletal muscle embryonic, HEMHC, MYHC-EMB, MYHSE1, SMHCE
Protein Accession Number:
NP_002461
Protein Gi Number:
98986453
Ncbi Gene Id Number:
4621
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Myosins are actin-based motor proteins that function in the generation of mechanical force in eukaryotic cells (1). MYH3 (myosin, heavy chain, skeletal muscle, embryonic) plays a significant role in skeletal muscle development (2) and is also essential for the proper morphology and function of the developing heart (3). Mutations in this gene have been associated with Freeman-Sheldon syndrome and Sheldon-Hall syndrome (4).
Background Reference 1:
Yu H, Waddell JN, Kuang S, et al. Park7 expression influences myotube size and myosin expression in muscle. PLoS One 2014; 9:e92030.
Background Reference 2:
Lagrutta AA, McCarthy JG, Scherczinger CA, et al. Identification and developmental expression of a novel embryonic myosin heavy-chain gene in chicken. DNA 1989; 8:39-50.
Background Reference 3:
Rutland CS, Polo-Parada L, Ehler E, et al. Knockdown of embryonic myosin heavy chain reveals an essential role in the morphology and function of the developing heart. Development 2011; 138:3955-66.
Background Reference 4:
Toydemir RM, Rutherford A, Whitby FG, et al. Mutations in embryonic myosin heavy chain (MYH3) cause Freeman-Sheldon syndrome and Sheldon-Hall syndrome. Nat. Genet. 2006; 38:561-5.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

8087

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

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