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Western blot analysis of FOXG1 in rat brain tissue lysate with FOXG1 antibody at 1 μg/ml.
Immunofluorescence of FOXG1 in mouse brain tissue with FOXG1 antibody at 20 μg/ml.
Western blot analysis of FOXG1 in rat brain tissue lysate with FOXG1 antibody at 1 μg/ml.
Trial Size

FOXG1 Antibody

CATALOG NUMBER: 8089

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Homology:
Predicted species reactivity based on immunogen sequence: Chicken: (100%)
Immunogen:
FOXG1 antibody was raised against an 18 amino acid peptide near the center of human FOXG1.
The immunogen is located within amino acids 160 - 210 of FOXG1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, WB
Application Note:
FOXG1 antibody can be used for detection of FOXG1 by Western blot at 1 - 2 μg/ml. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in rat samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Specificity:
FOXG1 antibody is human, mouse and rat reactive.
Positive Control 1:
Cat. No. 1463 - Rat Brain Tissue Lysate
Predicted Molecular Weight:
Predicted: 54 kDa
Observed: 54 kDa
Purification:
FOXG1 antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
FOXG1 antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
FOXG1 antibody can be stored at 4°C, stable for up to one year.
Ncbi Official Symbol:
FOXG1
Additional Names:
Forkhead box G1, Brain factor 1, Brain factor 2, BF1, BF2, FKH2, FKHL1, FKHL2, FKHL3, FKHL4, Forkhead box protein G1, FOXG1A, FOXG1B, FOXG1C, HBF-1, HBF2, HBF-3, HBF-G2, HFK1, HFK2, HFK3, KHL2, QIN
Protein Accession Number:
NP_005240
Protein Gi Number:
32307177
Ncbi Gene Id Number:
2290
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
FOXG1 belongs to the forkhead family of transcription factors which is characterized by a distinct forkhead domain and plays an important role in the development of the brain and telencephalon (1). It is essential for the proliferation of progenitor cells in the cerebral cortex and influences regional patterning in the mammalian telencephalon (2,3). Expression is restricted to the neurons of the developing telencephalon (3). The loss of FOXG1 results in an accelerated rate of neuronal differentiation and the shortening of the neurogenetic period in the embryonic cerebral cortex (2,4).
Background Reference 1:
Murphy DB, Wiese S, Burfeind P, et al. Human brain factor 1, a new member of the fork head gene family. Genomics 1994; 21:551-7.
Background Reference 2:
Kumamoto T, Toma K, Gunadi, et al. Foxg1 coordinates the switch from nonradially to radially migrating glutamatergic subtypes in the neocortex through spatiotemporal repression. Cell Rep. 2013; 3:931-45.
Background Reference 3:
Miyoshi G and Fishell G. Dynamic FoxG1 expression coordinates the integration of multipolar pyramidal neuron precursors into the cortical plate. Neuron 2012;74:1045-58.
Background Reference 4:
Mencarelli MA, Spanhol-Rosseto A, Artuso R, et al. Novel FOXG1 mutations associated with the congenital variant of Rett syndrome. J. Med. Genet. 2010; 47:49-53.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

8089

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

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