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Western blot analysis of SLC29A4 in SK-N-SH cell lysate with SLC29A4 antibody at 1 μg/ml.
Immunohistochemistry of SLC29A4 in human brain tissue with SLC29A4 antibody at 5 μg/mL.
Immunofluorescence of SLC29A4 in human brain tissue with SLC29A4 antibody at 20 μg/mL.
Western blot analysis of SLC29A4 in SK-N-SH cell lysate with SLC29A4 antibody at 1 μg/ml.
Trial Size

SLC29A4 Antibody

CATALOG NUMBER: 8131

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Mouse: (94%)
Immunogen:
SLC29A4 antibody was raised against an 18 amino acid peptide near the center of human SLC29A4.
The immunogen is located within amino acids 310 - 360 of SLC29A4.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
SLC29A4 antibody can be used for detection of SLC29A4 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificity:
SLC29A4 antibody is human specific. SLC29A4 antibody is predicted to not cross-react with other SLC29 proteins.
Positive Control 1:
Cat. No. 1220 - SK-N-SH Cell Lysate
Positive Control 2:
Cat. No. 10-301 - Human Brain Tissue Slide
Predicted Molecular Weight:
Predicted: 58 kDa
Observed: 58 kDa
Purification:
SLC29A4 antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
SLC29A4 antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
SLC29A4 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Ncbi Official Symbol:
SLC29A4
Additional Names:
Solute carrier family 29 member 4, Equilibrative nucleoside transporter 4, ENT4, PMAT
Protein Accession Number:
NP_001035751
Protein Gi Number:
100913034
Ncbi Gene Id Number:
222962
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
SLC29A4 is a member of the equilibrative nucleoside transporter family which plays a key role in nucleoside and nucleobase uptake for salvage pathways of nucleotide synthesis (1,2). SLC29A4 is a transmembrane protein that catalyzes the reuptake of monoamines into presynaptic neurons, thus determining the intensity and duration of monoamine neural signaling (1,3). SLC29A4 has been shown to transport several compounds, including serotonin, dopamine, and the neurotoxin 1-methyl-4-phenylpyridinium (3).
Background Reference 1:
Engel K, Zhou M, and Wang J. Identification and characterization of a novel monomine transporter in the human brain. J. Biol. Chem. 2004; 279:50042-9.
Background Reference 2:
Young JD, Yao SY, Baldwin JM, et al. The human concentrative and equilibrative nucleoside transporter families, SLC28 and SLC29. Mol. Aspects. Med. 34:529-47.
Background Reference 3:
Duan H and Wang J. Selective transport of monoamine neurotransmitters by human plasma membrane monoamine transporter and organic cation transporter 3. J. Pharmacol. Exp. Ther. 2010; 335:743-53.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

8131

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

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