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Western blot analysis of FAM212A in EL4 cell lysate with FAM212A antibody at (A) 1 and (B) 2 μg/ml.
Western blot analysis of FAM212A in EL4 cell lysate with FAM212A antibody at (A) 1 and (B) 2 μg/ml.
Trial Size

FAM212A Antibody

CATALOG NUMBER: 5531

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
FAM212A antibody was raised against a 15 amino acid peptide near the carboxy terminus of human FAM212A.
The immunogen is located within the last 50 amino acids of FAM212A.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
FAM212A antibody can be used for detection of FAM212A by Western blot at 1 - 2 μg/ml.
Antibody validated: Western Blot in mouse samples. All other applications and species not yet tested.
Specificity:
FAM212A antibody is human and mouse . At least two isoforms of FAM212A are known to exist; this antibody will recognize both isoforms.
Positive Control 1:
Cat. No. 1287 - EL4 Cell Lysate
Predicted Molecular Weight:
Predicted: 32 kDa
Observed: 33 kDa
Purification:
FAM212A antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
FAM212A antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
FAM212A antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Ncbi Official Symbol:
FAM212A
Additional Names:
Family with sequence similarity 212 member A, INKA1, C3orf54
Protein Accession Number:
NP_976248
Protein Gi Number:
42766424
Ncbi Gene Id Number:
389119
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
FAM212A, initially identified as INCA (induced in neural crest by AP-2alpha) in xenopus, is expressed primarily in neural crest cells and their derivatives (1). FAM212A can associate with the P21-kinase protein PAK4 and this association modulates cytoskeletal dynamics (1). In mammals, FAM212A is thought to play a role in neural tube closure; a significant fraction of FAM212A-null mice exhibited exencephaly (2).
Background Reference 1:
Luo T, Xu Y, Hoffman TL, et al. Inca: a novel p21-activated kinase-associated protein required for cranial neural crest development. Dev. 2007; 134:1279-89.
Background Reference 2:
Reid BS, Sargent TD, and Willams T. Generation and characterization of a novel neural crest marker allele, Inka1-LacZ, reveals a role for Inka1 in mouse neural tube closure. Dev. Dyn. 2010; 239:1188-96.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

5531

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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