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Western blot analysis of WSB1 in human colon tissue lysate with WSB1 antibody at 1 μg/ml.
Immunohistochemistry of WSB1 in human colon tissue with WSB1 antibody at 5 μg/mL.
Immunofluorescence of WSB1 in human colon tissue with WSB1 antibody at 20 μg/mL.
Western blot analysis of WSB1 in human colon tissue lysate with WSB1 antibody at 1 μg/ml.
Trial Size

WSB1 Antibody

CATALOG NUMBER: 8161

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Mouse: (82%)
Immunogen:
WSB1 antibody was raised against a 16 amino acid peptide near the amino terminus of human WSB1.
The immunogen is located within amino acids 70 - 120 of WSB1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
WSB1 antibody can be used for detection of WSB1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificity:
WSB1 antibody is human specific.
Positive Control 1:
Cat. No. 1320 - Human Colon Tissue Lysate
Predicted Molecular Weight:
Predicted: 46 kDa
Observed: 46 kDa
Purification:
WSB1 antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
WSB1 antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
WSB1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Ncbi Official Symbol:
WSB1
Additional Names:
WD repeat and SOCS box containing 1, SOCS box-containing WD protein SWiP-1, SWIP1, WD repeat and SOCS box-containing protein 1, WSB-1
Protein Accession Number:
NP_056441
Protein Gi Number:
18677720
Ncbi Gene Id Number:
26118
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The WD repeat and SOCS box-containing 1 (WSB1, also known as SWIP1), is a member of the WD-protein subfamily (1). It contains six WD-repeats spanning most of the protein and an SOCS box in the C-terminus and expressed as multiple alternatively spliced isoforms (1,2). WSB1 is thought to function as part of an SCF-like ECS (Elongin-Cullin-SOCS-box protein) E3 ubiquitin ligase complex that mediates the ubiquitination and proteasomal degradation of target proteins, such as DIO2 (3). Overexpression of WSB1 is implicated in pancreatic cancer progression, suggesting a role for WSB1 in carcinogenesis (4,5).
Background Reference 1:
Vasiliauskas D, Hancock S, Stern CD. SWiP-1: novel SOCS box containing WD-protein regulated by signalling centres and by Shh during development. Mech. Dev. 1999; 82:79-94.
Background Reference 2:
Nara H, Onoda T, Rahman M, et al. WSB-1, a novel IL-21 receptor binding molecule, enhances the maturation of IL-21 receptor. Cell Immunol. 2011; 269:54-9.
Background Reference 3:
Lv W, Zhang Y, Wu Z, et al. Identification of WSB1 gene as an important regulator in the development of zebrafish embryo during midblastula transition. Acta Biochim. Biophys. Sin (Shanghai) 2008; 40:478-88.
Background Reference 4:
Archange C, Nowak J, Garcia S, et al. The WSB1 gene is involved in pancreatic cancer progression. PLoS One 2008; 3:e2475.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

8161

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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