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Western blot analysis of UBD in human testis tissue lysate with UBD antibody at 1 μg/ml.
Immunohistochemistry of UBD in human testis tissue with UBD antibody at 5 μg/mL.
Immunofluorescence of UBD in human testis tissue with UBD antibody at 20 μg/ml.
Western blot analysis of UBD in human testis tissue lysate with UBD antibody at 1 μg/ml.
Trial Size

UBD Antibody

CATALOG NUMBER: 8185

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Rat: (81%)
Immunogen:
UBD antibody was raised against a 16 amino acid peptide near the center of human UBD.
The immunogen is located within amino acids 80 - 130 of UBD.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
UBD antibody can be used for detection of UBD by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificity:
UBD antibody is human specific.
Positive Control 1:
Cat. No. 1313 - Human Testis Tissue Lysate
Positive Control 2:
Cat. No. 11-701 - Human Testis Tissue Slide
Predicted Molecular Weight:
Predicted: 18 kDa
Observed: 17 kDa
Purification:
UBD antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
UBD antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
UBD antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Ncbi Official Symbol:
UBD
Additional Names:
Ubiquitin D, Diubiquitin, FAT10, GABBR1, UBD-3, Ubiquitin-like protein FAT10
Protein Accession Number:
NP_006389
Protein Gi Number:
222352096
Ncbi Gene Id Number:
10537
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
UBD, also designated ubiquitin D or diubiquitin, plays a role in antigen presentation, cytokine response and apoptosis. It is a 165 amino acid protein encoded in the major histocompatibility complex (MHC) that consists of two domains which share significant homology with ubiquitin (1,2). UBD is inducible by interferon gamma and TNF-alpha and regulates TNF-alpha-induced and LPS-mediated activation of the central mediator of innate immunity NF-kappa-B by promoting TNF-alpha-mediated proteasomal degradation of ubiquitinated-I-kappa-B-alpha (3). It may also regulate cell growth during dendritic cell or B cell activation and development (4,5).
Background Reference 1:
Ocklenburg F, Moharregh-Khiabani D, Geffers R, et al. UBD, a downstream element of FOXP3, allows the identification of LGALS3, a new marker of human regulatory T cells. Lab. Invest. 2006; 86:724-37.
Background Reference 2:
Cook WJ, Jeffrey LC, Carson M, et al. Structure of a diubiquitin conjugate and a model for interaction with ubiquitin conjugating enzyme (E2). J. Biol. Chem. 1992; 267:16467-71.
Background Reference 3:
Rahighi S, Ikeda F, Kawasaki M, et al. Specific recognition of linear ubiquitin chains by NEMO is important for NF-kappaB activation. Cell 2009;136:1098-109.
Background Reference 4:
Cort L, Habib M, Eberwine RA, et al. Diubiquitin (Ubd) is a susceptibility gene for virus-triggered autoimmune diabetes in rats. Genes Immun. 2014; 15:168-75.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

8185

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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