Start Your Project

🛍️Explore our Promotions page to discover current deals, limited-time offers, and more!🌟

Western blot analysis of PACS1 in rat bladder tissue lysate with PACS1 antibody at 1 μg/ml.
Immunohistochemistry of PACS1 in mouse brain tissue with PACS1 antibody at 2 μg/ml.
Immunofluorescence of PACS1 in human bladder tissue with PACS1 antibody at 20 μg/mL.
Immunofluorescence of PACS1 in mouse brain tissue with PACS1 antibody at 20 μg/ml.<br><br>Green: PACS1 Antibody (8187) <br> Blue: DAPI staining
Western blot analysis of PACS1 in rat bladder tissue lysate with PACS1 antibody at 1 μg/ml.
Trial Size

PACS1 Antibody

CATALOG NUMBER: 8187

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
PACS1 antibody was raised against a 17 amino acid peptide near the center of human PACS1.
The immunogen is located within amino acids 430 - 480 of PACS1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
PACS1 antibody can be used for detection of PACS1 by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 2 μg/mL For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in rat samples; Immunohistochemistry in mouse samples and Immunofluorescence in human and mouse samples. All other applications and species not yet tested.
Specificity:
PACS1 antibody is human, mouse and rat reactive. Multiple isoforms of PACS1 are known to exist. PACS1 antibody is predicted to not cross-react with PACS2.
Positive Control 1:
Cat. No. 1478 - Rat Bladder Tissue Lysate
Predicted Molecular Weight:
Predicted: 106 kDa
Observed: 105 kDa
Purification:
PACS1 antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PACS1 antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
PACS1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Ncbi Official Symbol:
PACS1
Additional Names:
Phosphofurin acidic cluster sorting protein 1, PACS-1, MRD17
Protein Accession Number:
NP_060496
Protein Gi Number:
30089916
Ncbi Gene Id Number:
55690
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The phosphofurin acidic cluster sorting protein 1 (PACS1), belongs to the PACS family of cytosolic connector proteins that localize different membrane proteins to the trans-Golgi network (TGN) (1,2). Mutations in PACS1 cause defects in cranial-neural crest migration during early development, leading to a recognizable intellectual disability syndrome (3). PACS1, along with the related protein PACS2, also plays a role in HIV-1 Nef-mediated downregulation of cell surface MHC-I molecules to the TGN, thereby enabling HIV-1 to escape immune surveillance (4).
Background Reference 1:
Wan L, Molloy SS, Thomas L, et al. PACS-1 defines a novel gene family of cytosolic sorting proteins required for trans-Golgi network localization. Cell 1998; 94:205-16.
Background Reference 2:
Kottgen M, Benzing T, Simmen T, et al. Trafficking of TRPP2 by PACS proteins represents a novel mechanism of ion channel regulation. EMBO J. 2005; 24:705-16.
Background Reference 3:
Schuurs-Hoeijmakers JH, Oh EC, Vissers LE, et al. Recurrent de novo mutations in PACS1 cause defective cranial-neural-crest migration and define a recognizable intellectual-disability syndrome. Am. J. Hum. Genet. 2012; 91:1122-7.
Background Reference 4:
Dikeakos JD, Thomas L, Kwon G, et al. An interdomain binding site on HIV-1 Nef interacts with PACS-1 and PACS2 on endosomes to down-regulate MHC-I. Mol. Biol. Cell 2012; 23:2184-97.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

8187

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.

Customize your order

Bulk Pricing Available

Custom Formulations: azide free, endotoxin testing & endotoxin removal.

Pair your antibody with a Lysate, Peptide, Control, & /OR Secondary Antibody.

Contact our Customer Care Team for more information

Custom Antibody Services

Speak to one of our custom antibody specialists to discuss your next custom antibody project.

New & Featured Products

HIV-1 p24 Antibody [9D2F6] (HRP)
HIV-1 p24 Antibody [9D2F6] (HRP)

CATALOG NUMBER: PM-7369-HRP

HIV-1 p24 Antibody [9D2F6] (biotin)
HIV-1 p24 Antibody [9D2F6] (biotin)

CATALOG NUMBER: PM-7369-biotin

HIV-1 p24 Antibody [9D2F6]
HIV-1 p24 Antibody [9D2F6]

CATALOG NUMBER: PM-7369