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Western blot analysis of PDIA1 in rat small intestine tissue lysate with PDIA1 antibody at (A) 1 and (B) 2 μg/ml.
Immunohistochemistry of PDIA1 in rat small intestine tissue with PDIA1 antibody at 5 μg/mL.
Immunofluorescence of PDIA1 in rat small intestine tissue with PDIA1 antibody at 20 μg/mL.
Western blot analysis of PDIA1 in rat small intestine tissue lysate with PDIA1 antibody at (A) 1 and (B) 2 μg/ml.
Trial Size

PDIA1 Antibody

CATALOG NUMBER: 8213

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Homology:
Predicted species reactivity based on immunogen sequence: Rabbit: (100%), Bovine: (100%), Chicken: (88%)
Immunogen:
PDIA1 antibody was raised against a 17 amino acid peptide near the center of human PDIA1.
The immunogen is located within amino acids 190 - 240 of PDIA1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
PDIA1 antibody can be used for detection of PDIA1 by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in rat samples; Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested.
Specificity:
PDIA1 antibody is human, mouse and rat reactive.
Positive Control 1:
Cat. No. 1468 - Rat Small Intestine Tissue Lysate
Predicted Molecular Weight:
Predicted: 54 kDa
Observed: 54 kDa
Purification:
PDIA1 antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PDIA1 antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
PDIA1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Ncbi Official Symbol:
P4HB
Additional Names:
Protein disulfide isomerase family A member 1, DSI, GIT, PDI, PHDB, PO4DB, PO4HB, PROHB, ERBA2L, P4Hbeta
Protein Accession Number:
NP_000909
Protein Gi Number:
20070125
Ncbi Gene Id Number:
5034
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
PDIA1 (protein disulfide isomerase family A member 1) is the beta subunit of prolyl 4-hydroxylase, a highly abundant multifunctional enzyme that belongs to the protein disulfide isomerase family. When present as a tetramer consisting of two alpha and two beta subunits, this enzyme is involved in hydroxylation of prolyl residues in preprocollagen (1,2). PDIA1 is also a disulfide isomerase containing two thioredoxin domains that catalyze the formation, breakage and rearrangement of disulfide bonds (3). Other known functions include its ability to act as a chaperone that inhibits aggregation of misfolded proteins in a concentration-dependent manner, its ability to bind thyroid hormone, its role in both the influx and efflux of S-nitrosothiol-bound nitric oxide, and its function as a subunit of the microsomal triglyceride transfer protein complex (4).
Background Reference 1:
Pihlajaniemi T, Helaakoski T, Tasanen K, et al. Molecular cloning of the beta-subunit of human prolyl 4-hydroxylase. This subunit and protein disulphide isomerase are products of the same gene. EMBO J. 1987; 6L:643-9.
Background Reference 2:
Walmsley AR, Batten MR, Lad U, et al. Intracellular retention of procollagen within the endoplasmic reticulum is mediated by prolyl 4-hydroxylase. J. Biol. Chem. 1999; 274:14884-92.
Background Reference 3:
Koivu J, Myllyla R, Halaakoski T, et al. A singel polypeptide acts both as the beta subunit of prolyl 4-hydroxylase and as a protein disulfide-isomerase. J. Biol. Chem. 1987; 262:6447-9.
Background Reference 4:
Kivirikko KI and Myllyharju J. Prolyl 4-hydroxylases and their protein disulfide isomerase subunit. Matrix Biol. 1998; 16:357-68.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

8213

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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