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Western blot analysis of TRPC3 in human cerebellum tissue lysate with TRPC3 antibody at (A) 1 and (B) 2 μg/ml.
Immunohistochemistry of TRPC3 in human brain tissue with TRPC3 antibody at 2.5 μg/mL.
Western blot analysis of TRPC3 in human cerebellum tissue lysate with TRPC3 antibody at (A) 1 and (B) 2 μg/ml.
Trial Size

TRPC3 Antibody

CATALOG NUMBER: 8269

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
TRPC3 antibody was raised against a 19 amino acid peptide from near the carboxy terminus of human TRPC3.
The immunogen is located within amino acids 710 - 760 of TRPC3.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC-P, WB
Application Note:
TRPC3 antibody can be used for the detection of TRPC3 by Western blot at 1 - 2 μg/mL.Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL.
Antibody validated: Western Blot in human samples and Immunohistochemistry in human samples. All other applications and species not yet tested.
Specificity:
TRPC3 antibody is human, mouse and rat reactive. At least four isoforms are known to exist; this antibody will detect the three largest isoforms. TRPC3 antibody is predicted to not cross-react with TRPC6.
Positive Control 1:
Cat. No. 1362 - Human Cerebellum Tissue Lysate
Positive Control 2:
Cat. No. 10-301 - Human Brain Tissue Slide
Predicted Molecular Weight:
Predicted: 90, 93, 101 kDa
Observed: 93 kDa
Purification:
TRPC3 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
TRPC3 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
TRPC3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Ncbi Official Symbol:
TRPC3
Additional Names:
TRP3, TRP3, Short transient receptor potential channel 3, Transient receptor protein 3, TrpC3
Protein Accession Number:
NP_001124170
Protein Gi Number:
194733735
Ncbi Gene Id Number:
7222
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The mammalian transient receptor potential (TRP) superfamily can be divided into three major families including the "canonical TRP" (TRPC) family. The seven members of this family share the activation through PLC-coupled receptors and have been suggested to be components of receptor-regulated cation channels in different cell types. Furthermore, the members of the TRPC3/6/7 subfamily can be activated by diacylglycerol analogs, suggesting a possible mechanism of activation of these channels by PLC-coupled receptors. TRPC3 encodes a Ca2+-permeant channel that is agonist-activated but not store-operated or directly receptor-activated. TRPC3 physically interacts with TRPC6 and TRPC7 and forms functional tetrameric channels.
Background Reference 1:
Contell C, Birnbaumer V, Flockerzi V, et al. A unified nomenclature for the superfamily of TRP cation channels. Mol. Cell 2002; 9:229-31.
Background Reference 2:
Trebak M, Vazquez G, Bird GSJ, et al. The TRPC3/6/7 subfamily of cation channels. Cell Calcium 2003; 33:451-61.
Background Reference 3:
Zitt C, Obukhov AG, Strubing C, et al. Expression of TRPC3 in Chinese hamster ovary cells results in calcium-activated cation currents not related to store depletion. J. Cell. Biol. 1997; 1333-41.
Background Reference 4:
Dietrich A, Kalwa H, Rost BR, et al. The diacylglycerol-sensitive TRPC3/6/7 subfamily of cation channels: functional characterization and physiological relevance. Pflugers Arch. 2005; 451:72-80.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

8269

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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