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Western blot analysis of Hexokinase 1 in rat bladder tissue lysate with Hexokinase 1 antibody at (A) 1 and (B) 2 μg/ml.
Immunohistochemistry of Hexokinase 1 in rat stomach tissue with Hexokinase 1 antibody at 5 μg/mL.
Immunofluorescence of Hexokinase 1 in rat stomach tissue with Hexokinase 1 antibody at 20 μg/mL.
Western blot analysis of Hexokinase 1 in rat bladder tissue lysate with Hexokinase 1 antibody at (A) 1 and (B) 2 μg/ml.
Trial Size

Hexokinase 1 Antibody

CATALOG NUMBER: 8217

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (94%)
Immunogen:
Hexokinase 1 antibody was raised against a 16 amino acid peptide near the carboxy terminus of human Hexokinase 1.
The immunogen is located within amino acids 830 - 880 of Hexokinase 1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
Hexokinase 1 antibody can be used for detection of Hexokinase 1 by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in rat samples; Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested.
Specificity:
Hexokinase 1 antibody is human, mouse and rat reactive. Multiple isoforms of Hexokinase 1 are known to exist.
Positive Control 1:
Cat. No. 1478 - Rat Bladder Tissue Lysate
Predicted Molecular Weight:
Predicted: 101 kDa
Observed: 103 kDa
Purification:
Hexokinase 1 antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
Hexokinase 1 antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
Hexokinase 1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Ncbi Official Symbol:
HK1
Additional Names:
HK, HXK
Protein Accession Number:
NP_000179
Protein Gi Number:
188497754
Ncbi Gene Id Number:
3098
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
There are four major glucose-phosphorylating isoenzymes, designated Hexokinase 1 I, II, III, and IV (1). Hexokinase 1 activity is involved in the first step in several metabolic pathways including phosphorylation of glucose to produce glucose-6-phosphate, thus committing glucose to the glycolytic pathway (1,2). Hexokinase 1 2 is the predominant Hexokinase 1 isozyme expressed in insulin-responsive tissues such as skeletal muscle and its expression is insulin-responsive (3). It is involved in the increased rate of glycolysis seen in rapidly growing cancer cells (4).
Background Reference 1:
Chen T, Ning D, Sun H, et al. Sequence Analysis and Molecular Characterization of Clonorchis sinensis Hexokinase 1, an Unusual Trimeric 50-kDa Glucose-6-Phosphate-Sensitive Allosteric Enzyme. PLoS One 2014; 9:e107940.
Background Reference 2:
Halestrap AP, Pereira GC, and Pasdois P. The role of Hexokinase 1 in cardioprotection-mechanism and potential for translation. Br. J. Pharmacol. 2014; epub.
Background Reference 3:
Roberts DJ and Miyamoto S. Hexokinase 1 II integrates energy metabolism and cellular protection: Akting on mitochondria and TORCing to autophagy. Cell Death Differ. 2014; epub.
Background Reference 4:
Wang L, Xiong H, Wu F, et al. Hexokinase 1 2-Mediated Warburg Effect Is Required for PTEN- and p53-Deficiency-Driven Prostate Cancer Growth. Cell Rep. 2014; 8:1461-74.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

8217

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

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