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Western blot analysis of RNF121 in MCF7 cell lysate with RNF121 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.
Immunocytochemistry of RNF121 in MCF7 cells with RNF121 antibody at 10 μg/ml.
Western blot analysis of RNF121 in MCF7 cell lysate with RNF121 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.
Trial Size

RNF121 Antibody

CATALOG NUMBER: 8285

Clonality:
Polyclonal
Tested Applications:
ELISA, ICC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
RNF121 antibody was raised against a 19 amino acid peptide near the carboxy terminus of human RNF121.
The immunogen is located within amino acids 260 - 310 of RNF121.
Conjugate:
Unconjugated
Tested Applications:
ELISA, ICC, WB
Application Note:
RNF121 antibody can be used for detection of RNF121 by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunocytochemistry at 10 μg/ml.
Antibody validated: Western Blot in human samples and Immunocytochemistry in human samples. All other applications and species not yet tested.
Specificity:
RNF121 antibody is human, mouse and rat reactive. Multiple isoforms of RNF121 are known to exist.
Positive Control 1:
Cat. No. 1219 - MCF7 Cell Lysate
Positive Control 2:
Cat. No. 17-012 - MCF7 Cell Slide
Predicted Molecular Weight:
Predicted: 24, 27, 36 kDa
Observed: 28 kDa
Purification:
RNF121 antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
RNF121 antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
RNF121 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Ncbi Official Symbol:
RNF121
Additional Names:
Ring finger protein 121
Protein Accession Number:
NP_060790
Protein Gi Number:
21361732
Ncbi Gene Id Number:
552978
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
RNF121 is an evolutionarily conserved E3 ligase ring finger protein that is expressed in the endoplasmic reticulum (1). It acts downstream of the unfolded protein response regulator protein kinase-like endoplasmic reticulum kinase (PERK). Overexpression of RNF121 during the early stages of gonad development led to aberrations in germline development and gonad migration, suggesting that it plays a role in endoplasmic reticulum-associated degradation machinery (1).
Background Reference 1:
Darom A, Bening-Abu-Shach U, and Broday L. RNF-121 is an endoplasmic reticulum-membrane E3 ubiquitin ligase involved in the regulation of B-integrin. Mol. Biol. Cell 2010; 21:1788-98.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

8285

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

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