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Western blot analysis of UBAP2L in HeLa cell lysate with UBAP2L antibody at 1 μg/ml.
Immunocytochemistry of UBAP2L in HeLa cells with UBAP2L antibody at 10 μg/ml.
Immunofluorescence of UBAP2L in HeLa cells with UBAP2L antibody at 20 μg/ml.<br><br>Red: UBAP2L Antibody (8371) <br> Blue: DAPI staining
Western blot analysis of UBAP2L in HeLa cell lysate with UBAP2L antibody at 1 μg/ml.
Trial Size

UBAP2L Antibody

CATALOG NUMBER: 8371

Clonality:
Polyclonal
Tested Applications:
ELISA, ICC, IF, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
UBAP2L antibody was raised against a 19 amino acid peptide from near the amino terminus of human UBAP2L.
The immunogen is located within amino acids 200 - 250 of UBAP2L.
Conjugate:
Unconjugated
Tested Applications:
ELISA, ICC, IF, WB
Application Note:
UBAP2L antibody can be used for the detection of UBAP2L by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry at 10 μg/ml.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificity:
UBAP2L antibody is human and mouse reactive. At least four isoforms of UBAP2L are known to exist; this antibody will detect all four isoforms. This antibody is predicted to not cross-react with UBAP2.
Positive Control 1:
Cat. No. 1201 - HeLa Cell Lysate
Positive Control 2:
Cat. No. 17-001 - HeLa Cell Slide
Predicted Molecular Weight:
Predicted: 120 kDa
Observed: 130 kDa
Purification:
UBAP2L Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
UBAP2L Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
UBAP2L antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Ncbi Official Symbol:
UBAP2L
Additional Names:
Ubiquitin associated protein 2-like, NICE-4
Protein Accession Number:
NP_055662
Protein Gi Number:
188497758
Ncbi Gene Id Number:
9898
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The UBA domain-containing protein ubiquitin associated protein 2-like (UBAP2L), also known as NICE4, is a ubiquitously expressed 1,087 amino acid protein. UBAP2L is preferentially expressed in hematopoietic stem cell (HSC) populations and interacts with BMI1, a well-known determinant of HSC function (1). It is essential for the activity of long-term repopulating HSCs but not the BMI1-dependent repression of the Ink4a/Arf locus (1).
Background Reference 1:
Bordeleau ME, Aucagne R, Chagraoui J, et al. UBAPL2L is a novel BMI1-iteracting protein essential for hematopoietic stem cell activity. Blood 2014; 124:2362-9.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

8371

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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