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Western blot analysis of Chondroadherin in 3T3 cell lysate with Chondroadherin antibody at 1 μg/ml.
Immunocytochemistry of Chondroadherin in 3T3 cells with Chondroadherin antibody at 5 μg/ml.
Western blot analysis of Chondroadherin in 3T3 cell lysate with Chondroadherin antibody at 1 μg/ml.
Trial Size

Chondroadherin Antibody

CATALOG NUMBER: 8421

Clonality:
Polyclonal
Tested Applications:
ELISA, ICC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (94%)
Immunogen:
Chondroadherin antibody was raised against a 17 amino acid peptide near the carboxy terminus of human Chondroadherin.
The immunogen is located within the last 50 amino acids of Chondroadherin.
Conjugate:
Unconjugated
Tested Applications:
ELISA, ICC, WB
Application Note:
Chondroadherin antibody can be used for detection of Chondroadherin by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunocytochemistry starting at 5 μg/mL.
Antibody validated: Western Blot in mouse samples and Immunocytochemistry in mouse samples. All other applications and species not yet tested.
Specificity:
Chondroadherin antibody is human and mouse reactive. Chondroadherin antibody is predicted to not cross-react with CHADL protein.
Positive Control 1:
Cat. No. 1210 - HEK293 Cell Lysate
Positive Control 2:
Cat. No. 17-201 - 3T3/BALB Cell Slide
Predicted Molecular Weight:
Predicted: 38 kDa
Observed: 36 kDa
Purification:
Chondroadherin antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
Chondroadherin antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
Chondroadherin antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Ncbi Official Symbol:
CHAD
Additional Names:
Cartilage leucine-rich protein, CHAD, SLRR4A
Protein Accession Number:
NP_001258
Protein Gi Number:
153251229
Ncbi Gene Id Number:
1101
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Chondroadherin, also known as CHAD or SLRR4A, belongs to the small leucine-rich proteoglycan (SLRP) family and class IV subfamily and plays a significant role in the regulation of chondrocyte growth and proliferation (1,2). It localizes to the extracellular matrix and contains nine tandem leucine repeats as well as N-terminal and C-terminal leucine rich domains (2-4). Chondroadherin is implicated in the adhesion of fibroblasts, osteoblasts and chondrocytes, which is mediated by interactions with Integrin alpha2 beta1 (2,4,5).
Background Reference 1:
Neame PJ, Sommarin Y, Boynton RE, et al. The structure of a 38-kDa leucine-rich protein (chondroadherin) isolated from bovine cartilage. J. Biol. Chem. 1994; 269:21547-54.
Background Reference 2:
Haglund L, Tillgren V, Addis L, et al. Identification and characterization of the integrin alpha2beta1 binding motif in chondroadherin mediating cell attachment. J. Biol. Chem. 2011; 286:3925-34.
Background Reference 3:
Mansson B, Wenglen C, Morgelin M, et al. Association of chondroadherin with collagen type II. J. Biol. Chem. 2001; 276:32883-8.
Background Reference 4:
Camper L, Heinegard D, and Lundgren-Akerlund E. Integrin alpha2beta1 is a receptor for the cartilage matrix protein chondroadherin. J. Cell Biol. 1997; 138:1159-67.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

8421

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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