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Western blot analysis of BHLHA15 in 3T3 cell lysate with BHLHA15 antibody at (A) 1 and (B) 2 μg/mL.
Western blot analysis of BHLHA15 in 3T3 cell lysate with BHLHA15 antibody at (A) 1 and (B) 2 μg/mL.
Trial Size

BHLHA15 Antibody

CATALOG NUMBER: 8541

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
BHLHA15 antibody was raised against a peptide corresponding to 17 amino acids near the carboxy terminus of human BHLHA15.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
BHLHA15 antibody can be used for Western blot at 1 - 2 μg/mL.
Antibody validated: Western Blot in mouse samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1282 - 3T3 (NIH) Cell Lysate
Predicted Molecular Weight:
Predicted: 21 kDa
Observed: 22 kDa
Purification:
BHLHA15 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
BHLHA15 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
BHLHA15 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
BHLHA15
Additional Names:
BHLHA15 Antibody: FB22, HM89, LAP3, LCR1, NPYR, WHIM, CD184, LESTR, NPY3R, NPYRL, HSY3RR, NPYY3R, D2S201E
Protein Accession Number:
NP_803238
Protein Gi Number:
29126247
Ncbi Gene Id Number:
168620
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
BHLHA15 Antibody: BHLHA15 (basic helix-loop-helix protein 15), also known as MIST1 (muscle intestine and stomach expression 1) belongs to the bHLH family of transcription factors and plays a role in regulating the transcriptional activity of MYOD1 in muscle cell development as well as serving as a key regulator of acinar cell function (1,2). BHLHA15 contains a basic helix-loop-helix (bHLH) domain and is capable of binding to E-box motifs as a homodimer or a heterodimer with E-proteins. It may also negatively regulate bHLH-mediated transcription through a N-terminal repressor domain (3). It is expressed in mammary epithelial cells and is essential for the regulation of mammary gland development (4).
Background Reference 1:
Lemercier C, To RQ, Swanson BJ et al. MIST1: a novel basic helix-loop-helix transcription factor exhibits a developmentally regulated expression pattern. Dev. Biol. 1997; 182:101-13.
Background Reference 2:
Pin CL, Rukstalis JM, Johnson C, et al. The bHLH transcription factor MIST1 is required to maintain exocrine pancreas cell organization and acinar cell identity. J. Cell Biol. 2001; 155:519-30.
Background Reference 3:
Lemercier C, To RQ, Carrasco RA et al. The basic helix-loop-helix transcription factor Mist1 functions as a transcriptional repressor of myoD. EMBO J. 1998; 17:1412-22.
Background Reference 4:
Zhao Y, Johansson C, Tran T, et al. Identification of a basic helix-loop-helix transcription factor expressed in mammary gland alveolar cells and required for maintenance of the differentiated state. Mol. Endocrinol. 2006; 20:2187-98.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

8541

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

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