Start Your Project

🛍️Explore our Promotions page to discover current deals, limited-time offers, and more!🌟

<strong>Figure 1 Western Blot Validation in Human and Mouse Cell Lines</strong><br>Loading: 15 μg of lysates per lane.Antibodies: SIGLEC15, 9017 (2 μg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
<strong>Figure 2 Western Blot Validation in Human Tissues</strong><br>Loading: 15 μg of lysates per lane.Antibodies: SIGLEC15, 9017 (2 μg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
<strong>Figure 3 Western Blot Validation in Mouse Tissues</strong><br>Loading: 15 μg of lysates per lane.Antibodies: SIGLEC15, 9017 (2 μg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
<strong>Figure 4 Western Blot Validation in Rat Tissues</strong><br>Loading: 15 μg of lysates per lane.Antibodies: SIGLEC15, 9017 (2 μg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Figure 1 Western Blot Validation in Human and Mouse Cell Lines
Loading: 15 μg of lysates per lane.Antibodies: SIGLEC15, 9017 (2 μg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
New
Trial Size

SIGLEC15 Antibody

CATALOG NUMBER: 9017

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Anti-SIGLEC15 antibody (9017) was raised against a peptide corresponding to 14 amino acids near the carboxy terminus of human SIGLEC15.
The immunogen is located within amino acids 270-320 of SIGLEC15.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
WB: 2 μg/mL.
Antibody validated: Western Blot in human, mouse and rat samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1201 - HeLa Cell Lysate
Positive Control 2:
Cat. No. 1211 - HepG2 Cell Lysate
Positive Control 3:
Cat. No. 1220 - SK-N-SH Cell Lysate
Positive Control 4:
Cat. No. 1304 - Human Liver Lysate
Positive Control 5:
Cat. No. 1404 - Mouse Liver Lysate
Positive Control 6:
Cat. No. 1463 - Rat brain Lysate
Predicted Molecular Weight:
Predicted: 36kD
Observed: 34-36kD
Isoforms:
Human SIGLEC15 has 2 isoforms, including isoform 1 (328aa, 36kD) and isoform 2 (174aa, 18kD). Mouse SIGLEC15 has one isoform (342aa, 37kD) and Rat SIGLEC15 also has one isoform (341aa, 37kD). 9017 can detect human and possibly mouse and rat isoforms.
Purification:
SIGLEC15 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
SIGLEC15 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
SIGLEC15 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
SIGLEC15
Additional Names:
SIGLEC15 Antibody: CD33L3, HsT1361, SIGLEC-15, CD33L3, Sialic acid-binding Ig-like lectin 15, CD33 antigen-like 3, Siglec-15
Protein Accession Number:
NP_998767
Protein Gi Number:
47106069
Ncbi Gene Id Number:
284266
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
SIGLEC15 Antibody: Siglecs are vertebrate cell-surface lectins that recognize sialylated glycans and are involved in many physiological processes, such as glycoprotein turnover, cellular trafficking, and pathogen recognition. Most Siglecs are expressed on cells of the immune system. SIGLEC15 is a type-I transmembrane protein consisting of two immunoglobulin (Ig)-like domains, a transmembrane domain containing a lysine residue, and a short cytoplasmic tail. SIGLEC15 can interact with the activating adaptor molecules DAP12/10. Its activating signaling potential and unique preference for glycan recognition implies that SIGLEC15 may be involved in the immune surveillance of tumors and probably plays a conserved, regulatory role in the immune system of vertebrates.
Background Reference 1:
Crocker and Redelinghuys. Biochem. Soc. Trans. 2008; 36:1467-71.
Background Reference 2:
Angata et al. Glycobiology 2007; 17:838-46.
Background Reference 3:
Hiruma et al. Biochem. Biophys. Res. Commun. 2011; 409:424-9.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

9017

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.

Customize your order

Bulk Pricing Available

Custom Formulations: azide free, endotoxin testing & endotoxin removal.

Pair your antibody with a Lysate, Peptide, Control, & /OR Secondary Antibody.

Contact our Customer Care Team for more information

Custom Antibody Services

Speak to one of our custom antibody specialists to discuss your next custom antibody project.

New & Featured Products

HIV-1 p24 Antibody [9D2F6] (HRP)
HIV-1 p24 Antibody [9D2F6] (HRP)

CATALOG NUMBER: PM-7369-HRP

HIV-1 p24 Antibody [9D2F6] (biotin)
HIV-1 p24 Antibody [9D2F6] (biotin)

CATALOG NUMBER: PM-7369-biotin

HIV-1 p24 Antibody [9D2F6]
HIV-1 p24 Antibody [9D2F6]

CATALOG NUMBER: PM-7369