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<strong>Figure 1 Western Blot Validation with SARS-CoV-2 (COVID-19) ORF9c Overexpressed 293 Cells</strong><br>Loading: 10 μg per lane of 293 cell lysate from WT and SARS-CoV-2 (COVID-19) ORF9c transfected cells.Antibodies: SARS-CoV-2 (COVID-19) ORF9c 9291, 1 μg/ml, 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
<strong>Figure 2 Western Blot Validation with SARS-CoV-2 (COVID-19) ORF9c Overexpressed 293 Cells</strong><br>Loading: 10 μg per lane of 293 cell lysate from WT and SARS-CoV-2 (COVID-19) ORF9c transfected cells.Antibodies: SARS-CoV-2 (COVID-19) ORF9c, 9291, 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.Lane 1: 0.25 μg/mL and Lane 2: 0.5 μg/mL
<strong>Figure 3 ELISA Validation </strong><br>Antibodies: SARS-CoV-2 (COVID-19) ORF9c Antibody, 9291. A direct ELISA was performed using SARS-CoV-2 ORF9c immunogen peptide (9291P)) as coating antigen and the anti-SARS-CoV-2 (COVID-19) ORF9c antibody as the capture antibody. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:20000 dilution. Detection range is from 0.3 ng/mL to 1000 ng/mL
Figure 1 Western Blot Validation with SARS-CoV-2 (COVID-19) ORF9c Overexpressed 293 Cells
Loading: 10 μg per lane of 293 cell lysate from WT and SARS-CoV-2 (COVID-19) ORF9c transfected cells.Antibodies: SARS-CoV-2 (COVID-19) ORF9c 9291, 1 μg/ml, 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
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SARS-CoV-2 (COVID-19) ORF9c Antibody

CATALOG NUMBER: 9291

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Virus
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Virus
Immunogen:
Anti-SARS-CoV-2 (COVID-19) ORF9c antibody (9291) was raised against a peptide corresponding to 16 amino acids near the amino-terminus of SARS-CoV-2 (COVID-19) ORF9c protein.
The immunogen is located within the first 50 amino acids of the SARS-CoV-2 (COVID-19) ORF9c protein.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
WB: 0.25-1 μg/mL
Antibody validated: SARS-CoV-2 (COVID-19) ORF9c antibody can detect 2 ng of free peptide at 1 μg/mL in ELISA. It can detect SARS-CoV-2 ORF9c in transfected human cells by WB. All other applications and species not yet tested
Specificity:
ORF9c Antibody is predicted to not cross-react with other coronavirus family members.
Predicted Molecular Weight:
8kD
Purification:
SARS-CoV-2 (COVID-19) ORF9c Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
SARS-CoV-2 (COVID-19) ORF9c antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
SARS-CoV-2 (COVID-19) ORF9c antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
ORF9c
Additional Names:
ORF9c protein, Uncharacterized protein 14, ORF14, ORF9c
Protein Accession Number:
P0DTD3
Protein Gi Number:
1835922084
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Coronavirus disease 2019 (COVID-19), formerly known as 2019-nCoV acute respiratory disease, is an infectious disease caused by SARS-CoV-2, a virus closely related to the SARS virus (1). The disease is the cause of the 2019–20 coronavirus outbreak (2). SARS-CoV-2 virus proteins include structural proteins, non-structural proteins and accessory factors. The structure of SARS-CoV-2 consists of the following: a spike protein (S), hemagglutinin-esterease dimer (HE), a membrane glycoprotein (M), an envelope protein (E) a nucleoclapid protein (N) and RNA. SARS-CoV-2 non-structural protein is ORF1ab that consists of 16 proteins (nsp1-nsp16), while accessory factors include ORF3a, ORF3b, ORF6, ORF7a, ORF7b, ORF8, ORF9b, ORF9c and ORF10. ORF9c may play a role in host-virus interaction.
Background Reference 1:
Gorbalenya. bioRxiv: 2020.
Background Reference 2:
Hui et al. Int J Infect Dis. 2020;91:264-266.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

9291

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

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