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Western blot analysis of ERGIC53 in HL60 cell lysate with ERGIC53 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking buffer.
Immunocytochemistry of ERGIC53 in HL60 cells with ERGIC53 antibody at 5 μg/ml.
Western blot analysis of ERGIC53 in HL60 cell lysate with ERGIC53 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking buffer.

ERGIC53 Monoclonal Antibody [1H6]

CATALOG NUMBER: PM-7213

Clonality:
Monoclonal
Tested Applications:
ELISA, ICC, WB
Host Species:
Mouse
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Mouse
Species Reactivity:
Human, Mouse
Immunogen:
Mouse monoclonal ERGIC53 antibody was raised against a 19 amino acid peptide near the carboxy terminus of human ERGIC53.
Conjugate:
Unconjugated
Tested Applications:
ELISA, ICC, WB
Application Note:
ERGIC53 antibody can be used for detection of ERGIC53 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 5 μg/mL.
Specificity:
At least two isoforms of ERGIC53 are known to exist.
Positive Control 1:
Cat. No. 1209 - HL60 Cell Lysate
Predicted Molecular Weight:
Predicted: 56 kDa
Purification:
ERGIC53 Monoclonal Antibody is Protein A purified.
Clonality:
Monoclonal
Isotype:
IgG1
physical-state:
Liquid
Buffer:
ERGIC53 Monoclonal Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
ERGIC53 Monoclonal antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Ncbi Official Symbol:
LMAN1
Additional Names:
ERGIC53 Monoclonal Antibody: Lectin mannose-binding 1, LMAN1, endoplasmic reticulum-golgi intermediate compartment protein 53, ERGIC-53, F5F8D, FMFD1, MCFD1, MR60, gp58
Protein Accession Number:
NP_005561
Protein Gi Number:
5031873
Ncbi Gene Id Number:
3998
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
ERGIC53 Monoclonal Antibody: ERGIC53, also known as lectin mannose-binding 1 (LMAN1) protein is a type I integral membrane protein localized in the intermediate region between the endoplasmic reticulum and the Golgi, presumably recycling between the two compartments. The protein is a mannose-specific lectin and is a member of a novel family of plant lectin homologs in the secretory pathway of animal cells. Mutations in the gene are associated with a coagulation defect. Using positional cloning, the gene was identified as the disease gene leading to combined factor V-factor VIII deficiency, a rare, autosomal recessive disorder in which both coagulation factors V and VIII are diminished.
Background Reference 1:
Schindler R, Itin C, Zerial M, et al. ERGIC-53, a membrane protein of the ER-Golgi intermediate compartment, carries an ER retention motif. Eur. J. Cell Biol. 1993; 61:1-9.
Background Reference 2:
ERGIC-53 is a functional mannose-selective and calcium-dependent human homologue of leguminous lectins. Mol. Biol Cell 1996; 7:483-93
Background Reference 3:
Zhang YC, Zhou Y, Yang CZ, et al. A review of ERGIC-53: its structure, functions, regulation and relations with diseases. Histol. Histopathol. 2009; 24:1193-204.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

PM-7213

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

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