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Western blot analysis of extracts of various cell lines, using TH antibody (13-009) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 15s.
Immunohistochemistry of paraffin-embedded rat brain using TH Antibody (13-009) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse spinal cord using TH Antibody (13-009) at dilution of 1:100 (40x lens).
Western blot analysis of extracts of various cell lines, using TH antibody (13-009) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 15s.

TH Antibody

CATALOG NUMBER: 13-009

Clonality:
Polyclonal
Tested Applications:
IF, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human TH (NP_954986.2).
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, WB
Application Note:
WB: 1:500 - 1:1000
IHC: 1:50 - 1:100
IF: 1:50 - 1:100
Positive Control 1:
Mouse brain
Positive Control 2:
Rat brain
Predicted Molecular Weight:
Observed: 59kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
TH
Additional Names:
TH, tyrosine hydroxylase, HGNC:11782, TYH, DYT14, DYT5b, dystonia 14, tyrosine 3-monooxygenase
Ncbi Gene Id Number:
7054
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene is involved in the conversion of tyrosine to dopamine. It is the rate-limiting enzyme in the synthesis of catecholamines, hence plays a key role in the physiology of adrenergic neurons. Mutations in this gene have been associated with autosomal recessive Segawa syndrome. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

13-009

List Size:
100 uL

List Price:

$436.00

Datasheet
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