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Western blot analysis of extracts of various cell lines, using SLC1A2 antibody (13-320) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 15s.
Immunohistochemistry of paraffin-embedded rat pancreas using SLC1A2 antibody (13-320) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver using SLC1A2 antibody (13-320) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse pancreas using SLC1A2 antibody (13-320) at dilution of 1:100 (40x lens).
Western blot analysis of extracts of various cell lines, using SLC1A2 antibody (13-320) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 15s.

SLC1A2 Antibody

CATALOG NUMBER: 13-320

Clonality:
Polyclonal
Tested Applications:
IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 495-574 of human SLC1A2 (NP_004162.2).
Conjugate:
Unconjugated
Tested Applications:
IHC, WB
Application Note:
WB: 1:500 - 1:5000
IHC: 1:100 - 1:200
Positive Control 1:
MCF7
Positive Control 2:
HepG2
Positive Control 3:
SW480
Positive Control 4:
Mouse liver
Positive Control 5:
Rat liver
Predicted Molecular Weight:
Observed: 65kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
SLC1A2
Additional Names:
EAAT2, EIEE41, GLT-1, HBGT, excitatory amino acid transporter 2, excitotoxic amino acid transporter 2, glutamate/aspartate transporter II, sodium-dependent glutamate/aspartate transporter 2, solute carrier family 1 (glial high affinity glutamate transporter), member 2
Ncbi Gene Id Number:
6506
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a member of a family of solute transporter proteins. The membrane-bound protein is the principal transporter that clears the excitatory neurotransmitter glutamate from the extracellular space at synapses in the central nervous system. Glutamate clearance is necessary for proper synaptic activation and to prevent neuronal damage from excessive activation of glutamate receptors. Improper regulation of this gene is thought to be associated with several neurological disorders. Alternatively spliced transcript variants of this gene have been identified.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

13-320

List Size:
100 uL

List Price:

$436.00

Datasheet
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