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Western blot analysis of extracts of various cell lines, using TGM2 antibody (13-350) at 1:300 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry of paraffin-embedded mouse brain using TGM2 antibody (13-350) (40x lens).
Immunohistochemistry of paraffin-embedded human lung cancer using TGM2 antibody (13-350) (40x lens).
Immunohistochemistry of paraffin-embedded human esophagus using TGM2 antibody (13-350) (40x lens).
Western blot analysis of extracts of various cell lines, using TGM2 antibody (13-350) at 1:300 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

TGM2 Antibody

CATALOG NUMBER: 13-350

Clonality:
Polyclonal
Tested Applications:
IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
Recombinant protein of human TGM2
Conjugate:
Unconjugated
Tested Applications:
IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:100 - 1:200
Positive Control 1:
SW480
Positive Control 2:
A-549
Positive Control 3:
DU145
Positive Control 4:
PC-3
Predicted Molecular Weight:
Observed: 90kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
TGM2
Additional Names:
TGM2, TG2, TGC, GNAH, HEL-S-45, G-ALPHA-h
Ncbi Gene Id Number:
7052
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Transglutaminases are enzymes that catalyze the crosslinking of proteins by epsilon-gamma glutamyl lysine isopeptide bonds. While the primary structure of transglutaminases is not conserved, they all have the same amino acid sequence at their active sites and their activity is calcium-dependent. The protein encoded by this gene acts as a monomer, is induced by retinoic acid, and appears to be involved in apoptosis. Finally, the encoded protein is the autoantigen implicated in celiac disease. Two transcript variants encoding different isoforms have been found for this gene.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

13-350

List Size:
100 uL

List Price:

$436.00

Datasheet
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