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Western blot analysis of extracts of various cell lines, using YAP1 antibody (13-379) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 10s.
Western blot analysis of extracts from normal (control) and YAP1 knockout (KO) HeLa cells, using YAP1 antibody (13-379) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human placenta using YAP1 antibody (13-379) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat kidney using YAP1 antibody (13-379) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse heart using YAP1 antibody (13-379) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of HeLa cells using YAP1 antibody (13-379) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of PC-12 cells using YAP1 antibody (13-370) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of PC12 cells using YAP1 antibody (13-379) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 200ug extracts of HeLa cells, using 3 ug YAP1 antibody (13-379). Western blot was performed from the immunoprecipitate using YAP1 antibody (13-379) at a dilition of 1:1000.
Western blot analysis of extracts of various cell lines, using YAP1 antibody (13-379) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s.
KO

YAP1 Antibody, KO Validated

CATALOG NUMBER: 13-379

Clonality:
Polyclonal
Tested Applications:
IF, IHC, IP, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 155-504 of human YAP1 (NP_001123617.1).
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, IP, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:50 - 1:200
IP: 1:20 - 1:50
Positive Control 1:
HeLa
Positive Control 2:
LO2
Positive Control 3:
BT-474
Positive Control 4:
A549
Predicted Molecular Weight:
Observed: 72kDa
Validation:
Antibody is Knockout validated.
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
YAP1
Additional Names:
YAP1, YAP, YAP2, YAP65, YKI
Ncbi Gene Id Number:
10413
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a downstream nuclear effector of the Hippo signaling pathway which is involved in development, growth, repair, and homeostasis. This gene is known to play a role in the development and progression of multiple cancers as a transcriptional regulator of this signaling pathway and may function as a potential target for cancer treatment. Alternative splicing results in multiple transcript variants encoding different isoforms.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

13-379

List Size:
100 uL

List Price:

$436.00

Datasheet
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