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Western blot analysis of extracts of various cell lines, using NACA antibody (13-442) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 10s.
Western blot analysis of extracts of various cell lines, using NACA antibody (13-442) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s.

NACA Antibody

CATALOG NUMBER: 13-442

Clonality:
Polyclonal
Tested Applications:
IHC, IP, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-215 of human NACA (NP_001106673.1).
Conjugate:
Unconjugated
Tested Applications:
IHC, IP, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:100
IP: 1:50 - 1:100
Positive Control 1:
HL-60
Positive Control 2:
SW620
Positive Control 3:
293T
Positive Control 4:
U-87MG
Positive Control 5:
HeLa
Positive Control 6:
Mouse liver
Predicted Molecular Weight:
Observed: 37kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
NACA
Additional Names:
HSD48, NAC-alpha1, skNAC, NACA, nascent polypeptide-associated complex subunit alpha, alpha-NAC, muscle-specific form, nascent-polypeptide-associated complex alpha polypeptide
Ncbi Gene Id Number:
4666
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a protein that associates with basic transcription factor 3 (BTF3) to form the nascent polypeptide-associated complex (NAC). This complex binds to nascent proteins that lack a signal peptide motif as they emerge from the ribosome, blocking interaction with the signal recognition particle (SRP) and preventing mistranslocation to the endoplasmic reticulum. This protein is an IgE autoantigen in atopic dermatitis patients. Alternative splicing results in multiple transcript variants, but the full length nature of some of these variants, including those encoding very large proteins, has not been determined. There are multiple pseudogenes of this gene on different chromosomes.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

13-442

List Size:
100 uL

List Price:

$436.00

Datasheet
Shipping Info

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