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Western blot analysis of extracts of various cell lines, using ZP2 antibody (13-444) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 10s.
Immunofluorescence analysis of rat oophoroma cells using ZP2 antibody (13-444) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of rat oophoroma cells using ZP2 antibody (13-444) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of mouse oophoroma cells using ZP2 antibody (13-444) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of mouse oophoroma cells using ZP2 antibody (13-444) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of mouse oophoroma cells using ZP2 antibody (13-444) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of mouse oophoroma cells using ZP2 antibody (13-444) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of mouse oophoroma cells using ZP2 antibody (13-444) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of mouse oophoroma cells using ZP2 antibody (13-444) at dilution of 1:100. Blue: DAPI for nuclear staining.
Western blot analysis of extracts of various cell lines, using ZP2 antibody (13-444) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s.

ZP2 Antibody

CATALOG NUMBER: 13-444

Clonality:
Polyclonal
Tested Applications:
IF, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 651-745 of human ZP2 (NP_003451.1).
Conjugate:
Unconjugated
Tested Applications:
IF, WB
Application Note:
WB: 1:1000 - 1:4000
IF: 1:50 - 1:200
Positive Control 1:
SH-SY5Y
Positive Control 2:
HL-60
Positive Control 3:
SW620
Positive Control 4:
Mouse liver
Positive Control 5:
Rat ovary
Predicted Molecular Weight:
Observed: 68kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
ZP2
Additional Names:
ZP2, ZPA, Zp-2
Ncbi Gene Id Number:
7783
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The zona pellucida is an extracellular matrix that surrounds the oocyte and early embryo. It is composed of three glycoproteins with various functions during fertilization and preimplantation development. The glycosylated mature peptide is one of the structural components of the zona pellucida and functions in secondary binding and penetration of acrosome-reacted spermatozoa. Female mice lacking this gene do not form a stable zona matrix and are sterile. Alternative splicing results in multiple transcript variants.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

13-444

List Size:
100 uL

List Price:

$436.00

Datasheet
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