Western blot analysis of extracts of A-431 cells, using BAGE2 antibody (13-472) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.

BAGE2 Antibody

CATALOG NUMBER: 13-472

Clonality:

Polyclonal

Tested Applications:

Host Species:

Rabbit

Species Reactivity:

Human

Conjugate:

Unconjugated

Specifications

Host Species:

Rabbit

Species Reactivity:

Human

Immunogen:

Recombinant fusion protein containing a sequence corresponding to amino acids 18-109 of human BAGE2 (NP_872288.2).

Conjugate:

Unconjugated

Tested Applications:

Application Note:

WB: 1:500 - 1:2000

Positive Control 1:

A-431

Predicted Molecular Weight:

Observed: 12kDa

Advanced Validation

Properties

Purification:

Affinity purification

Clonality:

Polyclonal

Isotype:

IgG

physical-state:

Liquid

Buffer:

PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Concentration:

batch dependent

Storage Conditions:

Store at -20°C. Avoid freeze / thaw cycles.

Additional Info

Ncbi Official Symbol:

BAGE2

Additional Names:

CT2.2, B melanoma antigen 2, B melanoma antigen family member 2, cancer/testis antigen 2.2, cancer/testis antigen family 2, member 2

Ncbi Gene Id Number:

85319

User Note:

Optimal dilutions for each application to be determined by the researcher.

Background

Citations

Set Contents

Disclaimer

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:

Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

13-472

List Size:

100 uL

List Price:

$419.00

Out of stock

Datasheet

Shipping Info

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$35 Dry Ice Shipment fee may be required on some items.

Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.

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BAGE2 Antibody

Clonality:

Tested Applications:

Host Species:

Species Reactivity:

Conjugate:

Host Species:

Species Reactivity:

Immunogen:

Conjugate:

Tested Applications:

Application Note:

Positive Control 1:

Predicted Molecular Weight:

Purification:

Clonality:

Isotype:

physical-state:

Buffer:

Concentration:

Storage Conditions:

Ncbi Official Symbol:

Additional Names:

Ncbi Gene Id Number:

User Note:

Disclaimer:

List Size:

Shipping Info

Customize your order

Custom Antibody Services

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