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Western blot analysis of extracts of various cell lines, using SLC4A5 antibody (13-648) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 15s.
Immunohistochemistry of paraffin-embedded rat liver using SLC4A5 antibody (13-648) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat kidney using SLC4A5 antibody (13-648) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver using SLC4A5 antibody (13-648) at dilution of 1:100 (40x lens).
Western blot analysis of extracts of various cell lines, using SLC4A5 antibody (13-648) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 15s.

SLC4A5 Antibody

CATALOG NUMBER: 13-648

Clonality:
Polyclonal
Tested Applications:
IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1042-1121 of human SLC4A5 (NP_597812.1).
Conjugate:
Unconjugated
Tested Applications:
IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
Positive Control 1:
LO2
Positive Control 2:
U-87MG
Positive Control 3:
SW480
Positive Control 4:
SGC-7901
Positive Control 5:
Mouse kidney
Predicted Molecular Weight:
Observed: 110kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
SLC4A5
Additional Names:
NBC4, NBCe2, electrogenic sodium bicarbonate cotransporter 4, solute carrier family 4 (sodium bicarbonate cotransporter), member 5
Ncbi Gene Id Number:
57835
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a member of the sodium bicarbonate cotransporter (NBC) family, part of the bicarbonate transporter superfamily. Sodium bicarbonate cotransporters are involved in intracellular pH regulation and electroneural or electrogenic sodium bicarbonate transport. This protein is thought to be an integral membrane protein. Multiple transcript variants encoding different isoforms have been found for this gene, but the biological validity of some variants has not been determined.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

13-648

List Size:
100 uL

List Price:

$436.00

Datasheet
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