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Western blot analysis of extracts of various cell lines, using TRMT61A antibody (13-751) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 10s.
Western blot analysis of extracts of various cell lines, using TRMT61A antibody (13-751) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s.

TRMT61A Antibody

CATALOG NUMBER: 13-751

Clonality:
Polyclonal
Tested Applications:
IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-289 of human TRMT61A (NP_689520.2).
Conjugate:
Unconjugated
Tested Applications:
IHC, WB
Application Note:
WB: 1:1000 - 1:2000
IHC: 1:50 - 1:200
Positive Control 1:
Jurkat
Positive Control 2:
BT-474
Positive Control 3:
U-251MG
Positive Control 4:
Mouse brain
Positive Control 5:
Mouse pancreas
Positive Control 6:
Mouse spleen
Predicted Molecular Weight:
Observed: 31kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
TRMT61A
Additional Names:
C14orf172, GCD14, Gcd14p, TRM61, hTRM61, tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRMT61A, mRNA methyladenosine-N(1)-methyltransferase catalytic subunit TRMT61A, tRNA (adenine-N(1)-)-methyltransferase catalytic subunit TRM61, tRNA (adenine-N(1)-)-methyltransferase catalytic subunit TRMT61A, tRNA methyltransferase 61 homolog A, tRNA(m1A58)-methyltransferase subunit TRM61, tRNA(m1A58)-methyltransferase subunit TRMT61A, tRNA(m1A58)MTase subunit TRM61, tRNA(m1A58)MTase subunit TRMT61A
Ncbi Gene Id Number:
115708
User Note:
Optimal dilutions for each application to be determined by the researcher.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

13-751

List Size:
100 uL

List Price:

$436.00

Datasheet
Shipping Info

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