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Western blot analysis of extracts of various cell lines, using DLG4 antibody (13-831).<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry of paraffin-embedded mouse cerebellum using DLG4 antibody (13-831) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat cerebellum using DLG4 antibody (13-831) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using DLG4 antibody (13-831) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of SH-SY-5Y cells using DLG4 antibody (13-831).
Immunofluorescence analysis of N2A cells using DLG4 antibody (13-831).
Western blot analysis of extracts of various cell lines, using DLG4 antibody (13-831).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

DLG4 Antibody [D5E5]

CATALOG NUMBER: 13-831

Clonality:
Monoclonal
Tested Applications:
IF, IHC, IP, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant protein of human DLG4
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, IP, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:50 - 1:200
IP: 1:20 - 1:50
Positive Control 1:
Mouse brain
Positive Control 2:
U-87MG
Predicted Molecular Weight:
Observed: 80kDa
Purification:
Affinity purification
Clonality:
Monoclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
DLG4
Additional Names:
synapse-associated protein 90, post-synaptic density protein 95, discs large homolog 4, Tax interaction protein 15, SAP90, PSD95, FLJ98574, FLJ97752, discs, large homolog 4 (Drosophila), DLG4
Ncbi Gene Id Number:
1742
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a member of the membrane-associated guanylate kinase (MAGUK) family. It heteromultimerizes with another MAGUK protein, DLG2, and is recruited into NMDA receptor and potassium channel clusters. These two MAGUK proteins may interact at postsynaptic sites to form a multimeric scaffold for the clustering of receptors, ion channels, and associated signaling proteins. Multiple transcript variants encoding different isoforms have been found for this gene.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

13-831

List Size:
50 uL

List Price:

$350.00

Datasheet
Shipping Info

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