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Western blot analysis of extracts of various cell lines, using ARHGEF7 antibody (13-905) at 1:500 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
Western blot analysis of extracts of various cell lines, using ARHGEF7 antibody (13-905) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

ARHGEF7 Antibody

CATALOG NUMBER: 13-905

Clonality:
Polyclonal
Tested Applications:
IF, WB
Host Species:
Rabbit
Species Reactivity:
Human, Monkey, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Monkey, Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human ARHGEF7 (NP_001106984.1).
Conjugate:
Unconjugated
Tested Applications:
IF, WB
Application Note:
WB: 1:500 - 1:2000
IF: 1:50 - 1:100
Positive Control 1:
SW620
Positive Control 2:
Jurkat
Positive Control 3:
COS-1
Positive Control 4:
COS-7
Positive Control 5:
MCF7
Positive Control 6:
K-562
Predicted Molecular Weight:
Observed: 86kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
ARHGEF7
Additional Names:
Rho guanine nucleotide exchange factor 7, Beta-Pix, COOL-1, PAK-interacting exchange factor beta, p85, ARHGEF7, COOL1, KIAA0142, P85SPR, PAK3BP, PIXB
Ncbi Gene Id Number:
8874
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a protein that belongs to a family of cytoplasmic proteins that activate the Ras-like family of Rho proteins by exchanging bound GDP for GTP. It forms a complex with the small GTP binding protein Rac1 and recruits Rac1 to membrane ruffles and to focal adhesions. Multiple alternatively spliced transcript variants encoding different isoforms have been observed for this gene.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

13-905

List Size:
100 uL

List Price:

$436.00

Datasheet
Shipping Info

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