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Western blot analysis of extracts of various cell lines, using BBC3 antibody (14-003).<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry of paraffin-embedded mouse stomach using BBC3 antibody (14-003) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human mammary cancer using BBC3 antibody (14-003) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human gastric cancer using BBC3 antibody (14-003) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse intestin using BBC3 antibody (14-003) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of SKOV-3 cells using BBC3 antibody (14-003).
Immunofluorescence analysis of HeLa cells using BBC3 antibody (14-003).
Western blot analysis of extracts of various cell lines, using BBC3 antibody (14-003).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

BBC3 Antibody [A6G9]

CATALOG NUMBER: 14-003

Clonality:
Monoclonal
Tested Applications:
IF, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant protein of human BBC3
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:50 - 1:200
Positive Control 1:
HeLa
Positive Control 2:
K-562
Predicted Molecular Weight:
Observed: 18kDa
Purification:
Affinity purification
Clonality:
Monoclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
BBC3
Additional Names:
PUMA Antibody: JFY1, PUMA, JFY-1, Bcl-2-binding component 3
Ncbi Gene Id Number:
27113
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a member of the BCL-2 family of proteins. This family member belongs to the BH3-only pro-apoptotic subclass. The protein cooperates with direct activator proteins to induce mitochondrial outer membrane permeabilization and apoptosis. It can bind to anti-apoptotic Bcl-2 family members to induce mitochondrial dysfunction and caspase activation. Because of its pro-apoptotic role, this gene is a potential drug target for cancer therapy and for tissue injury. Alternative splicing results in multiple transcript variants.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

14-003

List Size:
50 uL

List Price:

$335.00

Out of stock

Datasheet
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