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Western blot analysis of extracts of various cell lines, using IKB alpha antibody (14-011) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
Western blot analysis of extracts from normal (control) and IKB alpha knockout (KO) 293T cells, using IKB alpha antibody (14-011) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 3s.
Immunoprecipitation analysis of 150ug extracts of A549 cells using 3ug IKB alpha antibody (14-011). Western blot was performed from the immunoprecipitate using IKB alpha antibody (14-011) at a dilition of 1:500.
Western blot analysis of extracts of various cell lines, using IKB alpha antibody (14-011) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
KO

IKB alpha Antibody, KO Validated

CATALOG NUMBER: 14-011

Clonality:
Polyclonal
Tested Applications:
IP, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-317 of human IKB alpha (NP_065390.1).
Conjugate:
Unconjugated
Tested Applications:
IP, WB
Application Note:
WB: 1:500 - 1:2000
IP: 1:20 - 1:50
Positive Control 1:
MCF-7
Positive Control 2:
DU145
Positive Control 3:
THP-1
Positive Control 4:
A-549
Positive Control 5:
Mouse spleen
Positive Control 6:
Mouse thymus
Predicted Molecular Weight:
Observed: 36kDa
Validation:
Antibody is Knockout validated.
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
NFKBIA
Additional Names:
NFKBIA, IKBA, MAD-3, NFKBI
Ncbi Gene Id Number:
4792
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a member of the NF-kappa-B inhibitor family, which contain multiple ankrin repeat domains. The encoded protein interacts with REL dimers to inhibit NF-kappa-B/REL complexes which are involved in inflammatory responses. The encoded protein moves between the cytoplasm and the nucleus via a nuclear localization signal and CRM1-mediated nuclear export. Mutations in this gene have been found in ectodermal dysplasia anhidrotic with T-cell immunodeficiency autosomal dominant disease.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

14-011

List Size:
100 uL

List Price:

$436.00

Datasheet
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