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Western blot analysis of extracts of various cell lines, using SNAIL antibody (14-144) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 60s.
Immunohistochemistry of paraffin-embedded rat kidney using SNAIL antibody (14-144) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver using SNAIL antibody (14-144) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using SNAIL antibody (14-144) at dilution of 1:100 (40x lens).
Western blot analysis of extracts of various cell lines, using SNAIL antibody (14-144) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 60s.

SNAIL Antibody

CATALOG NUMBER: 14-144

Clonality:
Polyclonal
Tested Applications:
IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-264 of human SNAIL (NP_005976.2).
Conjugate:
Unconjugated
Tested Applications:
IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
Positive Control 1:
HeLa
Positive Control 2:
293T
Positive Control 3:
MCF7
Positive Control 4:
DU145
Positive Control 5:
Mouse heart
Positive Control 6:
Mouse lung
Predicted Molecular Weight:
Observed: 34kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
SNAI1
Additional Names:
SNAI1, SNA, SNAH, SNAIL, SLUGH2, SNAIL1, dJ710H13.1
Ncbi Gene Id Number:
6615
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The Drosophila embryonic protein snail is a zinc finger transcriptional repressor which downregulates the expression of ectodermal genes within the mesoderm. The nuclear protein encoded by this gene is structurally similar to the Drosophila snail protein, and is also thought to be critical for mesoderm formation in the developing embryo. At least two variants of a similar processed pseudogene have been found on chromosome 2.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

14-144

List Size:
100 uL

List Price:

$436.00

Datasheet
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