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Western blot analysis of extracts of various cell lines, using ARHGDIA antibody (14-275) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
Western blot analysis of extracts from normal (control) and ARHGDIA knockout (KO) 293T cells, using ARHGDIA antibody (14-275) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 10s.
Immunofluorescence analysis of HeLa cells using ARHGDIA antibody (14-275). Blue: DAPI for nuclear staining.
Western blot analysis of extracts of various cell lines, using ARHGDIA antibody (14-275) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
KO

ARHGDIA Antibody, KO Validated

CATALOG NUMBER: 14-275

Clonality:
Polyclonal
Tested Applications:
IF, WB
Host Species:
Rabbit
Species Reactivity:
Human, Monkey, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Monkey, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-204 of human ARHGDIA (NP_001172006.1).
Conjugate:
Unconjugated
Tested Applications:
IF, WB
Application Note:
WB: 1:500 - 1:2000
IF: 1:50 - 1:200
Positive Control 1:
HeLa
Positive Control 2:
COS7
Positive Control 3:
PC-12
Positive Control 4:
Mouse lung
Positive Control 5:
Mouse spleen
Positive Control 6:
Mouse kidney
Predicted Molecular Weight:
Observed: 25kDa
Validation:
Antibody is Knockout validated.
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
ARHGDIA
Additional Names:
ARHGDIA, RHOGDI-1, RHOGDI, Rho GDP dissociation inhibitor (GDI) alpha, MGC117248, GDIA1
Ncbi Gene Id Number:
396
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a protein that plays a key role in the regulation of signaling through Rho GTPases. The encoded protein inhibits the disassociation of Rho family members from GDP (guanine diphosphate), thereby maintaining these factors in an inactive state. Activity of this protein is important in a variety of cellular processes, and expression of this gene may be altered in tumors. Mutations in this gene have been found in individuals with nephrotic syndrome, type 8. Alternate splicing results in multiple transcript variants.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

14-275

List Size:
100 uL

List Price:

$540.00

Datasheet
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