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Western blot analysis of extracts of various cell lines, using TOPBP1 antibody (14-518) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 30s.
Immunohistochemistry of paraffin-embedded mouse lung using TOPBP1 antibody (14-518) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat lung using TOPBP1 antibody (14-518) at dilution of 1:100 (40x lens).
Western blot analysis of extracts of various cell lines, using TOPBP1 antibody (14-518) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 30s.

TOPBP1 Antibody

CATALOG NUMBER: 14-518

Clonality:
Polyclonal
Tested Applications:
IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human TOPBP1 (NP_008958.2).
Conjugate:
Unconjugated
Tested Applications:
IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
Positive Control 1:
Jurkat
Positive Control 2:
K-562
Predicted Molecular Weight:
Observed: 171kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
TOPBP1
Additional Names:
Dpb11, TOP2BP1, DNA topoisomerase 2-binding protein 1, DNA topoisomerase II-beta-binding protein 1, topoisomerase (DNA) II binding protein 1
Ncbi Gene Id Number:
11073
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a binding protein which interacts with the C-terminal region of topoisomerase II beta. This interaction suggests a supportive role for this protein in the catalytic reactions of topoisomerase II beta through transient breakages of DNA strands.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

14-518

List Size:
100 uL

List Price:

$419.00

Out of stock

Datasheet
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