Start Your Project

🛍️Explore our Promotions page to discover current deals, limited-time offers, and more!🌟

Western blot analysis of extracts of various cell lines, using TEAD1 antibody (14-950) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 120s.
Immunohistochemistry of paraffin-embedded human lung cancer using TEAD1 antibody (14-950) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver cancer using TEAD1 antibody (14-950) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human colon carcinoma using TEAD1 antibody (14-950) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human esophageal cancer using TEAD1 antibody (14-950) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human gastric cancer using TEAD1 antibody (14-950) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse lung using TEAD1 antibody (14-950) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of MCF7 cells using TEAD1 antibody (14-950).
Chromatin immunoprecipitation analysis of extracts of HeLa cell line, using TEAD1 rabbit polyclonal antibody (14-950) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
Western blot analysis of extracts of various cell lines, using TEAD1 antibody (14-950) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 120s.

TEAD1 Antibody

CATALOG NUMBER: 14-950

Clonality:
Polyclonal
Tested Applications:
ChIP, IF, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 135-215 of human TEAD1 (NP_068780.2).
Conjugate:
Unconjugated
Tested Applications:
ChIP, IF, IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:50 - 1:200
CHIP: 1:20 - 1:50
Positive Control 1:
HeLa
Positive Control 2:
MCF-7
Positive Control 3:
Mouse kidney
Positive Control 4:
Mouse heart3
Predicted Molecular Weight:
Observed: 48kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
TEAD1
Additional Names:
TEAD1, AA, REF1, TCF13, TEF-1, NTEF-1, TCF-13, TEAD-1
Ncbi Gene Id Number:
7003
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a ubiquitous transcriptional enhancer factor that is a member of the TEA/ATTS domain family. This protein directs the transactivation of a wide variety of genes and, in placental cells, also acts as a transcriptional repressor. Mutations in this gene cause Sveinsson's chorioretinal atrophy. Additional transcript variants have been described but their full-length natures have not been experimentally verified.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

14-950

List Size:
100 uL

List Price:

$436.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.

Customize your order

Bulk Pricing Available

Custom Formulations: azide free, endotoxin testing & endotoxin removal.

Pair your antibody with a Lysate, Peptide, Control, & /OR Secondary Antibody.

Contact our Customer Care Team for more information

Custom Antibody Services

Speak to one of our custom antibody specialists to discuss your next custom antibody project.

New & Featured Products

HIV-1 p24 Antibody [9D2F6] (HRP)
HIV-1 p24 Antibody [9D2F6] (HRP)

CATALOG NUMBER: PM-7369-HRP

HIV-1 p24 Antibody [9D2F6] (biotin)
HIV-1 p24 Antibody [9D2F6] (biotin)

CATALOG NUMBER: PM-7369-biotin

HIV-1 p24 Antibody [9D2F6]
HIV-1 p24 Antibody [9D2F6]

CATALOG NUMBER: PM-7369