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Western blot analysis of extracts of various cell lines, using UPF2 antibody (14-975) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 60s.
Immunohistochemistry of paraffin-embedded rat kidney using UPF2 antibody (14-975) at dilution of 1:200 (40x lens).
Western blot analysis of extracts of various cell lines, using UPF2 antibody (14-975) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 60s.

UPF2 Antibody

CATALOG NUMBER: 14-975

Clonality:
Polyclonal
Tested Applications:
IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1153-1272 of human UPF2 (NP_542166.1).
Conjugate:
Unconjugated
Tested Applications:
IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
Positive Control 1:
MCF7
Positive Control 2:
HT-29
Positive Control 3:
Jurkat
Positive Control 4:
HepG2
Positive Control 5:
HeLa
Predicted Molecular Weight:
Observed: 180kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
UPF2
Additional Names:
UPF2, UPF2 regulator of nonsense transcripts homolog (yeast), HGNC:17854, DKFZP434D222, HUPF2, KIAA1408, RENT2, OTTHUMP00000045025, UPF2 regulator of nonsense transcripts homolog, regulator of nonsense transcripts 2, yeast Upf2p homolog
Ncbi Gene Id Number:
26019
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a protein that is part of a post-splicing multiprotein complex involved in both mRNA nuclear export and mRNA surveillance. mRNA surveillance detects exported mRNAs with truncated open reading frames and initiates nonsense-mediated mRNA decay (NMD). When translation ends upstream from the last exon-exon junction, this triggers NMD to degrade mRNAs containing premature stop codons. This protein is located in the perinuclear area. It interacts with translation release factors and the proteins that are functional homologs of yeast Upf1p and Upf3p. Two splice variants have been found for this gene; both variants encode the same protein.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

14-975

List Size:
100 uL

List Price:

$436.00

Datasheet
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