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Western blot analysis of extracts of various cell lines, using HSP90AA1 antibody (15-028) at 1:500 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
Immunoprecipitation analysis of 200ug extracts of HeLa cells using 1ug HSP90AA1 antibody (15-028). Western blot was performed from the immunoprecipitate using HSP90AA1 antibody (15-028) at a dilition of 1:500.
Western blot analysis of extracts of various cell lines, using HSP90AA1 antibody (15-028) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

HSP90AA1 Antibody

CATALOG NUMBER: 15-028

Clonality:
Polyclonal
Tested Applications:
IP, WB
Host Species:
Rabbit
Species Reactivity:
Human, Monkey, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Monkey, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 433-732 of human HSP90AA1 (NP_005339.3).
Conjugate:
Unconjugated
Tested Applications:
IP, WB
Application Note:
WB: 1:500 - 1:2000
IP: 1:20 - 1:50
Positive Control 1:
HeLa
Positive Control 2:
COS-1
Positive Control 3:
COS-7
Positive Control 4:
PC-12
Predicted Molecular Weight:
Observed: 101kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
HSP90AA1
Additional Names:
HSP90, HSP90A, HSPC1, HSPCA, Heat shock protein HSP 90-alpha, Heat shock 86 kDa, HSP 86, EL52, HSPN, LAP2, HSP86, Hsp89, Hsp90, HSP89A, HSP90N, HSPCAL1, HSPCAL4
Ncbi Gene Id Number:
3320
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene is an inducible molecular chaperone that functions as a homodimer. The encoded protein aids in the proper folding of specific target proteins by use of an ATPase activity that is modulated by co-chaperones. Two transcript variants encoding different isoforms have been found for this gene.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

15-028

List Size:
100 uL

List Price:

$436.00

Out of stock

Datasheet
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