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Western blot analysis of extracts of 293T cells, using SIN3A antibody (15-271) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat liver using SIN3A antibody (15-271) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human lung cancer using SIN3A antibody (15-271) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver cancer using SIN3A antibody (15-271) at dilution of 1:100 (40x lens).
Western blot analysis of extracts of 293T cells, using SIN3A antibody (15-271) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 90s.

SIN3A Antibody

CATALOG NUMBER: 15-271

Clonality:
Polyclonal
Tested Applications:
IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-120 of human SIN3A (NP_056292.1).
Conjugate:
Unconjugated
Tested Applications:
IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
Positive Control 1:
293T
Predicted Molecular Weight:
Observed: 147kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
SIN3A
Additional Names:
SIN3A, DKFZP434K2235, FLJ90319, KIAA0700,
Ncbi Gene Id Number:
25942
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene is a transcriptional regulatory protein. It contains paired amphipathic helix (PAH) domains, which are important for protein-protein interactions and may mediate repression by the Mad-Max complex.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

15-271

List Size:
100 uL

List Price:

$436.00

Datasheet
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