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Western blot analysis of extracts of various cell lines, using IFI16 antibody (15-315) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat intestine using IFI16 Antibody (15-315) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat lung using IFI16 Antibody (15-315) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat liver using IFI16 Antibody (15-315) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat kidney using IFI16 Antibody (15-315) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human kidney cancer using IFI16 Antibody (15-315) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse lung using IFI16 Antibody (15-315) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse liver using IFI16 Antibody (15-315) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse kidney using IFI16 Antibody (15-315) at dilution of 1:100 (40x lens).
Western blot analysis of extracts of various cell lines, using IFI16 antibody (15-315) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.

IFI16 Antibody

CATALOG NUMBER: 15-315

Clonality:
Polyclonal
Tested Applications:
IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human IFI16 (NP_005522.2).
Conjugate:
Unconjugated
Tested Applications:
IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
Positive Control 1:
Mouse spleen
Positive Control 2:
Mouse lung
Predicted Molecular Weight:
Observed: 88kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
IFI16
Additional Names:
IFNGIP1, PYHIN2, gamma-interferon-inducible protein 16, IFI16 beta isoform, interferon-gamma induced protein IFI 16, interferon-inducible myeloid differentiation transcriptional activator
Ncbi Gene Id Number:
3428
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a member of the HIN-200 (hematopoietic interferon-inducible nuclear antigens with 200 amino acid repeats) family of cytokines. The encoded protein contains domains involved in DNA binding, transcriptional regulation, and protein-protein interactions. The protein localizes to the nucleoplasm and nucleoli, and interacts with p53 and retinoblastoma-1. It modulates p53 function, and inhibits cell growth in the Ras/Raf signaling pathway. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

15-315

List Size:
100 uL

List Price:

$436.00

Datasheet
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