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Western blot analysis of extracts of various cell lines, using S100A10 antibody (15-656) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
Immunohistochemistry of paraffin-embedded rat ovary using S100A10 antibody (15-656) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human colon using S100A10 antibody (15-656) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human breast cancer using S100A10 antibody (15-656) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse testis using S100A10 antibody (15-656) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human breast cancer using S100A10 antibody (15-656) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse leydig cells using S100A10 antibody (15-656) at dilution of 1:100 (40x lens).
Western blot analysis of extracts of various cell lines, using S100A10 antibody (15-656) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.

S100A10 Antibody

CATALOG NUMBER: 15-656

Clonality:
Polyclonal
Tested Applications:
IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Monkey, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Monkey, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-97 of human S100A10 (NP_002957.1).
Conjugate:
Unconjugated
Tested Applications:
IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
Positive Control 1:
HT-29
Positive Control 2:
VERO
Positive Control 3:
HeLa
Positive Control 4:
A-549
Positive Control 5:
NIH/3T3
Predicted Molecular Weight:
Observed: 11kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
S100A10
Additional Names:
S100A10, 42C, ANX2LG, Calpactin-1 light chain, Calpactin I light chain, CLP11, p11, p10, p10 protein, Protein S100-A10, ANX2L, Ca[1], CAL1L, Cellular ligand of annexin II, gp11
Ncbi Gene Id Number:
6281
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in exocytosis and endocytosis.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

15-656

List Size:
100 uL

List Price:

$436.00

Datasheet
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